A procedure was developed to isolate and determine ergot alkaloid production by Acremonium coenophialum, the endophytic fungus of tall fescue. The procedure established that macerated leaf sheath or pith from inRorescence stem placed either in a liquid medium or on a corn meal-malt extract agar medium produced isolated mycelium and characteristic conidia within a 3to 3.5-week period. Once isola...

The aim of the study was to evaluate the anti-inflammatory activity of the S. paranense crude extract, S. paranense alkaloid fraction, and solanine alkaloid. These samples reduce the croton oil-induced ear edema in a dose-dependent manner and a maximum inhibition of 81%, 98%, and 80% in the doses of 1.0, 0.73, and 0.37 mg/ear, respectively. Moreover, the samples inhibit the MPO activity with an...

A procedure was developed to isolate and determine ergot alkaloid production by Acremonium coenophialum, the endophytic fungus of tall fescue. The procedure established that macerated leaf sheath or pith from inflorescence stem placed either in a liquid medium or on a corn meal-malt extract agar medium produced isolated mycelium and characteristic conidia within a 3- to 3.5-week period. Once is...

Background and objectives: Many members of the Amaryllidaceae are regarded as toxic. The toxic constituents that occur in the whole family are referred to as the Amaryllidaceae alkaloids. The main aim of this study was the identification of alkaloid compounds from Galanthus transcaucasicus Fomin, a medicinal plant from Amaryllidaceae. Methods: Planar and column chromatography techniques were us...

The alkaloid chemistry of the venom of hybrid fire ant, Solenopsis richteri × Solenopsis invicta, was investigated using silica gel chromatography and GC-MS techniques. In addition to most cis alkaloids of parental species, S. richteri Forel and S. invicta Buren, the cis alkaloid fraction of the body extract of hybrid fire ants also contains five significant new alkaloids. Hydrogenation of the ...

Five groups of six rabbits each were infected with 10 6 cell/ml inoculums of “enteropathogenic” Escherichia coli and treated with alkaloid extract of Phyllanthus niruri administered as strawberry at 100mg/day divided in four doses. Sample of serum and urine were collected before infection, 26hr after infection, 3 rd day of therapy (72hr) and 48hr after 7 th day of therapy. Creatinine, urea nitr...

Alkaloid contents in Argemone mexicana cell line AMMiF were analyzed during a 36-day culture cycle. The benzophenanthridine sanguinarine (SA) represented the main alkaloid. Dihydrosanguinarine (DHSA), a SA biosynthetic precursor and a less toxic benzophenanthridine, was also identified, based on chromatographic properties and further confirmed by gas chromatography coupled to mass spectrometry....

Plants are considered as rich resources of ingredients which can be used in drug development pharmacopoeia Medicinal, non-pharmacopoeial or synthetic drugs. 100g each plant part were extracted using different organic and aqueous solvents have varying polarity (Petroleum ether, EtOAc, EtOH,H20).The extract was filter the subjected to column tube. Compound from mixture compounds collected Alkaloi...

The immunomodulatory activity of diterpene alkaloids from Aconitum lycoctonum L. (Ranunculaceae family) was evaluated; the alkaloid components were extracted from plants grown wild in the Apennines near Parma (Northern Italy). The diterpene alkaloid lycoctonine evaluated by means of HPLC and IR techniques reaches its highest value in flowers, followed by stems, roots and leaves, while the maxim...

Many medicinal plants were with a long history of use in folk medicine against a variety of diseases. Recently, many researchers have taken a great interest on medicinal plants for their phytochemical constituents and biological activities including antioxidant activity. The present study was aimed to evaluate phytochemical analysis, quantification of total phenolic, alkaloid contents and antio...