نتایج جستجو برای: alui
تعداد نتایج: 302 فیلتر نتایج به سال:
The aim of this study was molecular identification of S. aureus strains isolated from mastitic milk samples and establishing the genetic relationship between strains isolated from cows belonging to the same herd. In all 43 isolated strains the gap gene (930 bp) was amplified, which enabled their affiliation to the Staphylococcus genus to be established. PCR-RFLP with AluI endonuclease of the ga...
Staphylococcus aureus is a major pathogen in the transmission of diseases from animals to humans and vice-versa.Various infections, such as mastitis in cattle, sheep and goats, as well as gastroenteritis due to food poisoning in humans are the most frequent problems caused by S. aureus. The bacteria also lead to severe economic losses in dairy industry. A major virulence facto...
Aiming to develop a DNA marker specific for Bacillus anthracis and able to discriminate this species from Bacillus cereus, Bacillus thuringiensis, and Bacillus mycoides, we applied the randomly amplified polymorphic DNA (RAPD) fingerprinting technique to a collection of 101 strains of the genus Bacillus, including 61 strains of the B. cereus group. An 838-bp RAPD marker (SG-850) specific for B....
Automated sequence analysis of a 410-bp region of the axyR gene in 105 Mycobacterium tuberculosis complex isolates identified a polymorphic nucleotide that differentiated Mycobacterium bovis isolates from other complex members. All 29 M. bovis isolates sequenced had an adenine residue at nucleotide 285, whereas all 76 other complex isolates had a guanine residue. PCR-restriction fragment length...
Phenotypic Performance and The Characterization of Growth Hormone (GH|AluI) in Bangkok Chicken Breed
This study aimed to determine Bangkok chicken’sphenotypic characteristics and growth hormone genes using the PCR-RFLP method. The samples used were 50 chickens. Research in field is taking phenotypic traits data. laboratory carried out by DNA isolation, amplification, restriction. showed that DOC weight,body weight, bodyweight gain of DOC-1 month, 1-2 months, 2-3 months chickens significantly d...
a total of 105 isolates of erysiphe betae from different regions of iran were investigated. for probable polymorphism of its-5.8s regions using pcr-based rflp, the mentioned fragments were amplified via pcr by its1 and its4 primers and subjected to 1.5% agarose gel electrophoresis. pcr products were digested by haeiii, alui, taqi, hindiii, cfoi, ecori, mspi, saci and rsai res and the digestion ...
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