Probestin has been isolated as part of a program designed to find microorganism-produced inhibitors of aminopeptidase M from Streptomyces azureus MH663-2F6. It was purified by use of column chromatography of Amberlite XAD-4, silica gel, YMC-gel, Toyopearl HW-40, YMC D-ODS-5 (HPLC) and then isolated as colorless powders. Probestin is competitive with the substrate, and the inhibition constant (K...

1. An Amberlite XAD-2 column in a heated (37 degrees C) jacket was incorporated in between two banks of bioassay organs superfused with Krebs bicarbonate solution in cascade. The column removed prostaglandins, E1, E2 and F2alpha and also rabbit aorta contracting substance (RCS) and possibly slow reacting substance of anaphylaxis (SRS-A). 2. The column gave free passage to histamine in concentra...

More than 1.0ƒÊg of 20ƒ¿-hydroxypregn-4-en-3-one and progesterone in the ovarian vein blood of rabbits can be quantitatively determined with a new chromatographic system using a partially esterified carboxylic acid type ion exchange resin, Amberlite IRC-50, and a mixture of ethanol and water (3:2 v/v) used as stationary phase and moving phase respectively. Before administration of human chorion...

This work describes a methodology for the valorization of non-food biomass production biosourced molecules via biohydrogen and metabolites fermentations such as volatile fatty acids. The commercial anion exchange resin Amberlite IRA-400(Cl) was chosen extraction carboxylic Different operating conditions were set up namely amount to be used, activation methods, pH their effects on capacity solve...

BACKGROUND The polyacrylic resin Amberlite IRA-67 is a promising adsorbent for lactic acid extraction from aqueous solution, but little systematic research has been devoted to the separation efficiency of lactic acid under different operating conditions. METHODOLOGY/PRINCIPAL FINDINGS In this paper, we investigated the effects of temperature, resin dose and lactic acid loading concentration o...

The amino acid composition of twice recrystallized pepsin (Worthington Biochemical Corporation) has been determined chromatographically on columns of Amberlite IR 120 resin. The results of the analyses obtained on four different preparations indicate a close agreement in their amino acid composition. Pepsin is unique in that it has a great predominance of acidic amino acids over basic ones. Mor...

A fraction enriched in the minor components of the kanamycin complex was prepared from a commercial sample. The enriched fraction was subjected to Amberlite CG-50 and Dowex 1-X2 column chromatography. Two minor components were isolated in addition to the known A, B, and C components. These congeners were identified as paromamine and 6-o-(3-amino-3-deoxy-alpha-d-glucopyranosyl)- deoxystreptamine...

A method is described for the automated analysis of urinary 11-deoxy-17-oxo steroids from hydrolyzed conjugate extracts. Steroid conjugates are extracted manually on an Amberlite XAD-2 column and hydrolyzed with f3-glucuronidase at 62#{176}C for 90 mm. After addition of alkali, the samples are transferred to the AutoAnalyzer where the steroids are extracted into isooctane and estimated colorime...