Hepatitis C Virus (HCV) encodes two envelope glycoproteins, E1 and E2. Our previous work selected a specific aptamer ZE2, which could bind to E2 with high affinity, with a great potential for developing new molecular probes as an early diagnostic reagents or therapeutic drugs targeting HCV. In this study, the binding sites between E2 and aptamer ZE2 were further explored. E2 was truncated to 15...

Ileal digesta were collected via a post-valve-T-caecum cannula from piglets ( lo-15 kg) fed diets based on raw pea or on pea protein isolate (Finale or Frijaune variety). From the determined amino acids (AA) content of feed and digesta, AA apparent digestibility coefficients and proportions of proteins (endogenous, bacterial, dietary) theoretically present in digesta were calculated. Four essen...

The purpose of this study was to look at the effects of an amino-acid mixture, highfat feeding and exercise on blood glucose and plasma insulin levels in skeletal muscle of female Sprague-Dawley rats. Rats were either fed a standard-diet only (SD-CON), a standard-diet plus a pre-established amino-acid mixture (CON-AA), a high-fat diet only (HF-CON), a high-fat diet plus a pre-established amino-...

background: dermatophagoides farinae and dermatophagoides pteronyssinus are house dust mites (hdm) that they cause severe asthma and allergic symptoms. tropomyosin protein plays an important role in mentioned immune and allergic reactions to hdms. here, tropomyosin protein from dermatophagoides spp. was comprehensively screened in silico for its allergenicity, antigenicity and similarity/conser...

Amino acid (AA) and fatty (FA) composition of the fertilised eggs different larval stages (at 0, 3, 6, 9, 12, 15 and21 days post-hatch, dph) Chanos chanos was investigated. The total indispensable amino acids (IAA) contributed to55.62% AA in egg which reduced to 52.54% on 6 dph. profile C. found be rich invaline (7.99%), leucine (7.51%) lysine (6.98%) poor histidine (2.36%) methionine (2.47%), ...

A fibrinogen fragment obtained by limited-plasmin proteolysis has been isolated and purified to apparent homogeneity by gel filtrations. This fragment, denoted as 24-Kd fragment, has an apparent M, -24,000 and contains an N-terminal sequence of met-glu-leu-glu-arg-pro-gly-glyasn-glu-ile. The fragment contains a large number of acidic amino acid residues, and its amino acid composition is simila...

BACKGROUND Polycystic ovary syndrome (PCOS) is a heterogeneous endocrine disorder frequently accompanied by obesity and by insulin resistance, and patients with this syndrome suffer from infertility and poor pregnancy outcome. Disturbances in plasma amino acid (AA) metabolism have been implicated in women with PCOS. However, direct evidence on follicular AA metabolic profiles in PCOS patients a...

A fibrinogen fragment obtained by limited-plasmin proteolysis has been isolated and purified to apparent homogeneity by gel filtrations. This fragment, denoted as 24-Kd fragment, has an apparent M, -24,000 and contains an N-terminal sequence of met-glu-leu-glu-arg-pro-gly-glyasn-glu-ile. The fragment contains a large number of acidic amino acid residues, and its amino acid composition is simila...

Mammalian translation initiation factor 4F (eIF4F) consists of three subunits, eIF4A, eIF4E, and eIF4G. eIF4G interacts directly with both eIF4A and eIF4E. The binding site for eIF4E is contained in the amino-terminal third of eIF4G, while the binding site for eIF4A was mapped to the carboxy-terminal third of the molecule. Here we show that human eIF4G possesses two separate eIF4A binding domai...

Abstract Amino acid (AA) was used as a green grafting agent to functionalize polyepoxysuccinic (PESA), and three AA-modified PESA (AA-PESA) CaCO3 scale inhibitors were obtained change the structural singleness of further improve its comprehensive properties. The structures AA-PESA characterized by Fourier transform infrared (FTIR) spectroscopy nuclear magnetic resonance hydrogen (1HNMR) spectro...