The paired helical filaments (PHFs) of Alzheimer's disease were purified by a strategy in which the neurons and amyloid plaque cores of protein (APCP) were initially isolated. This was achieved by several steps of isocratic sucrose centrifugations of increasing molarity and a discontinuous isotonic Percoll density gradient. After collagenase elimination of contaminating blood vessels, lysis of ...

It has been reported by Walker (1) and Fitch et al. (2) that kidneys from rats fed normal diets supplemented with creatine had lower transamidinase activities in vitro than kidneys from rats fed normal diets. It therefore seemed of interest to determine if the creatine-depressing effect could be prevented or reversed by the addition of certain compounds to creatine-containing diets. Also, it wa...

A new 2-amino imidazole derivative, 5-(4-chlorophenyl)-N,1-di-o-tolyl-1H-imidazole-2-amine (3), has been synthesized using a green approach. The reaction was conducted in ChCl (cholinium chloride)/urea eutectic mixture, which is nature-inspired and environmentally friendly medium. proposed mechanism involves the preliminary regioselective alkylation of Nsp2 guanidine (2), followed by an intramo...

New fluorescent compounds, benzo[4,5]thieno[3,2-d]pyrimidine 5,5-dioxides (3a-g), 2-amino-4-methylsulfanylbenzo[4,5]thieno[3,2-d]pyrimidine (6), and 2-amino-4-methylsulfanyl-7-methoxybenzo[4,5]furo[3,2-d]pyrimidine (7), were synthesized in good yields from heterocyclic ketene dithioacetals (1a-c) and guanidine carbonate (2a) or (S)-methylisothiourea sulfate (2b) in pyridine under reflux. Among ...

Contact-based antimicrobials, as antibiotic-free technologies that use non-specific interactions with bacterial cells to exert antimicrobial activity, are a prospective solution in fighting the global issue of resistance. A very simplified approach their design considers direct bonding cationic guanidine-containing amino acids surface nano-gold carriers. The structure enables activity due high ...

In studies on muscular dystrophy in young individuals investigations of the urine were made along three lines, as follows: (a) the estimation of creatinine and creatine, according to Folin (1) and Benedict (2) respectively; (b) the study as to whether the creatine findings include other substances than creatine; (c) the possibility that in muscular dystrophy guanidine intoxication plays a promi...

Inhibition of HeLa cell deoxyribonucleic acid (DNA) synthesis, which occurred by the 4th to 5th hr after infection with poliovirus, could be blocked completely by guanidine only when it was present before the 2nd hr. At the 2nd hr, there was no significant ribonucleic acid (RNA)-replicase activity, and addition of guanidine inhibited all production of virus but allowed 57% of maximal DNA inhibi...

The z-average molecular weight of horse liver alcohol dehydrogenase in dilute neutral buffer is 78,200 by sedimentation equilibrium. Guanidine hydrochloride, 1 M, reversibly inhibits enzymatic activity. The inhibition with nicotinamide adenine dinucleotide is competitive and with ethanol is mixed. When ethylenediaminetetraacetate is added to the enzyme in 1 M guanidine hydrochloride, zinc is re...