Introduction An economical way to obtain optically active β-lactam derivatives is the use of amino acids as chiral auxiliaries in Staudinger reaction (Bose et al., 1985). This strategy has been used for asymmetric synthesis of bicyclic β-lactam compounds, such as 2-isocephems, from threonine (Tenneson and Belleau, 1980). In addition, β-lactams obtained by this method are useful as versatile int...

Conservative treatment of chronic kidney disease, apart from simply treating symptoms and associated complications, consists in slowing disease progression, order to improve patient family quality life, postpone the need for renal replacement therapy reduce costs. Slowing progression involves therapeutic strategies, aiming avoid/treat malnutrition inflammation, correct anemia, treat mineral bon...

CYP719A14 structure analysis and its interaction with ligands via bioinformatic methods. Methods. Homologous modeling of three-dimensional enzyme. Molecular docking. Results. enzyme, which shows cheilanthifoline synthase activity, model was build based on results homologous modeling. Interaction built possible substrates – benzylisoquinoline compounds: (S)-Scoulerine, (S)-Cheilanthifoline, (S)-...

DinB (DNA Pol IV) is a translesion (TLS) DNA polymerase, which inserts a nucleotide opposite an otherwise replication-stalling N(2)-dG lesion in vitro, and confers resistance to nitrofurazone (NFZ), a compound that forms these lesions in vivo. DinB is also known to be part of the cellular response to alkylation DNA damage. Yet it is not known if DinB active site residues, in addition to aminoac...

New types of asymmetric functionalizations of alkenes are highly desirable for chemical synthesis. Here, we develop three novel types of regio- and enantioselective multiple oxy- and amino-functionalizations of terminal alkenes via cascade biocatalysis to produce chiral α-hydroxy acids, 1,2-amino alcohols and α-amino acids, respectively. Basic enzyme modules 1-4 are developed to convert alkenes...