The objectives of this study were to compare surgical techniques and the effects of using n-butyl 2cyanoacrylate and bovine amniotic membrane to repair perforated lesions in corneas. Penetrating keratoplasty was performed in sixty New Zealand White rabbits under general anesthesia. Group 1 (G1) was treated with n-butyl 2-cyanoacrylate, group 2 (G2) received a fragment of amniotic membrane throu...

The amniotic membrane is a tissue of fetal origin and is composed of three major layers: a single epithelial layer, a thick basement membrane, and an avascular mesenchyme [Fernandes et al., 2005]. There are no nerves, muscles or lymphatics in the amnion. The amniotic membrane is derived from the inner cell mass in the blastocyst and is adjacent to the trophoblast cells and lines the amniotic ca...

PURPOSE To evaluate how sutureless amniotic membrane patches may affect visual functions in normal human eyes. DESIGN Prospective intervention study. METHODS Ten sets of sutureless amniotic membrane patch manufactured as PROKERA were inserted in one eye of six normal patients. Four sets (one each) were inserted in four patients, while six sets (three each) were inserted in two patients. Unc...

The amniotic membrane is the innermost layer of fetal membrane and is attached to the chorion in the placenta. This membrane has been used for nearly a century in varied fields such as ophthalmology, reconstructive surgery, and burn treatment. In this case report, we used a human amniotic membrane to repair an iatrogenic oroantral communication that occurred during the extraction of the patient...

Background: Equine wounds, particularly those involving the distal portion of the limbs, often undergo prolonged complex healing and may enter a non-healing state with obvious financial and welfare implications. Poor blood supply and lower levels of oxygen to the lower limbs lead to imbalance of growth factors related to wound healing. Decellular amniotic membrane has been widely used in soft t...

UNLABELLED The new defined anatomical and functional complex conjunctiva-limbus-cornea is a new concept, which helps clinicians better understand and treat ocular surface pathologies. The management of the ocular surface disease has changed dramatically over the years, with spectacular improvements of techniques, and of course, results. The amniotic membrane, used as a graft or as a substrate f...

Denuded human amniotic membrane is widely proposed to be used for retinal pigment epithelial cell transplantation. Notch signaling though important and known to have role in both health and disease of retinal pigment epithelial cells; is not well studied for retinal pigment epithelial cells cultured on denuded human amniotic membrane. The outcome of Notch signals is strictly dependent on the ce...

OBJECTIVE To determine whether amniotic membrane transplantation can be used to treat symptomatic bullous keratopathy displaying poor visual potential. METHODS Amniotic membrane transplantation was performed at 5 centers on 50 consecutive eyes (50 patients) with symptomatic bullous keratopathy and poor visual potential. The underlying causes of bullous keratopathy included aphakia (9 eyes), p...

© Shanghai Jiao Tong University Press, Shanghai and Springer Science+Business Media Dordrecht 2015 R.C. Zhao (ed.), Stem Cells: Basics and Clinical Translation, Translational Medicine Research 1, DOI 10.1007/978-94-017-7273-0_2 Abstract Application of amnion membrane with multiple bioactive biomaterial has over 100 years of history. Amnion membraneand amniotic fluid-derived stem cells mainly in...