Amplified fragment length polymorphism (AFLP) was investigated for the differentiation of Corynebacterium diphtheriae isolates. Analysis using Taxotron revealed 10 distinct AFLP profiles among 57 isolates. Strains with ribotype patterns D1, D4, and D12 could not be distinguished; however, the technique discriminated isolates of ribotype patterns D3, D6, and D7 further. AFLP was rapid, fairly in...

We examined 154 Norwegian B. cereus and B. thuringiensis soil isolates (collected from five different locations), 8 B. cereus and 2 B. thuringiensis reference strains, and 2 Bacillus anthracis strains by using fluorescent amplified fragment length polymorphism (AFLP). We employed a novel fragment identification approach based on a hierarchical agglomerative clustering routine that identifies fr...

A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) test was performed to investigate the allele frequencies of the insulin-like growth factor (IGF1 and IGF2 genes) in 90 broilerchickens. A 793 bp fragment of IGF1 gene and 1146 bp fragment of IGF2 gene were amplified and digested with HinfI and Hsp92II restriction enzymes,respectively. Two types of alleles of A and B...

Amplified-fragment length polymorphism (AFLP) is a whole-genome fingerprinting method based on selective amplification of restriction fragments. The potential of the method for the characterization of mycoplasmas was investigated in a total of 50 strains of human and animal origin, including Mycoplasma genitalium (n = 11), Mycoplasma pneumoniae (n = 5), Mycoplasma hominis (n = 5), Mycoplasma hy...

A polymerase chain reaction-based DNA typing method, amplified fragment length polymorphism (AMP-FLP) analysis, has shown promise as a means of analyzing forensic biological evidence. A variety of DNA extraction methods were evaluated for their suitability for AMP-FLP analysis. Factors that were considered in the evaluation included DNA yield, ability of DNA to be amplified, the presence of DNA...

Amplified fragment length polymorphism (AFLP) analysis of botanical forensic evidence provides a means of obtaining a reproducible DNA profile in a relatively short period of time in species for which no sequence information is available. AFLP profiles were obtained for 40 Acer rubrum trees. Leaf material from five additional species was also typed. Genomic DNA was isolated using the DNeasy Pla...

P. H. M. SAVELKOUL,* H. J. M. AARTS, J. DE HAAS, L. DIJKSHOORN, B. DUIM, M. OTSEN, J. L. W. RADEMAKER, L. SCHOULS, AND J. A. LENSTRA Department of Medical Microbiology and Infection Control, University Hospital Vrije Universiteit, Amsterdam, DLO-State Institute of Quality Control of Agricultural Products Centre De Born, Wageningen, Novartis Seeds bv, Section Biotechnology, Enkhuizen, Department...

The silkworm (Bombyx mori L.) is a lepidopteran insect with a long history of significant agricultural value. We have constructed the first amplified fragment length polymorphism (AFLP) genetic linkage map of the silkworm B. mori at a LOD score of 2.5. The mapping AFLP markers were genotyped in 47 progeny from a backcross population of the cross no. 782 x od100. A total of 1248 (60.7%) polymorp...