ABSTRACT Aflatoxin biosynthesis was induced by compounds in filtrates (EF) obtained from cultures consisting of ground maize kernels colonized by Aspergillus flavus. The inducing activity increased to a maximum at 4 days of incubation and then decreased. Amylase activity was detected in the EF, suggesting that the inducers are products of starch degradation (glucose, maltose, and maltotriose). ...

  Tannins are plant-derived polyphenolic compounds that are widely found in foods, particularly in fruits and beverages. These materials are water-soluble and able to precipitate proteins from aqueous solutions. It had been shown that feeding some animals such as rat and mouse with tannins mimicks the effects of isoproterenol on salivary glands. Tannins stimulate β-receptors by some indirect me...

To evaluate the effectiveness of gibberellic acid (GA 3 ) in breaking rice seed dormancy and the use of a-amylase enzyme activity as an indicator of the dormancy level, seed from the intensively dormant irrigated cultivar Urucuia were used. The seeds were submitted to a predrying process in a forced air circulation chamber under 40oC during 7 days and submersed in 30 mL of GA 3 solution under 0...

The aims of study were to investigate the effects of intraperitoneal (i.p.) infusion of ghrelin on pancreatic α-amylase outputs and the responses of pancreatic proteins to ghrelin that may relate to the pancreatic exocrine. Six male Sprague-Dawley rats (300 g) were randomly divided into two groups, a control group (C, n = 3) and a treatment group (T, 10.0μg/kg BW, n = 3). Blood samples were col...

Food allergy from wheat is triggered by several protein classes, such as LTPs, ω5-gliadins and α-amylase/trypsin inhibitors. The latter proteins, belonging to the prolamin superfamily, are mostly involved in baker's asthma, a form of occupational allergy in which the sensitization occurs through the respiratory tract. α-Amylase/trypsin inhibitors were also found to be involved in wheat-related ...

The protein composition of oral fluid is modulated by environmental factors and physiological states, i.e. chemical, mechanical and pharmacologic stimuli, pathologic conditions, and psychological stress. Secretory protein concentrations in samples of whole saliva (WS) from children were measured and the results were subjected to statistical analysis. Protein expression was determined using elec...

We evaluated the polyethylene glycol precipitation test (Gastroenterology 83: 378-382, 1982), looking for macroamylase in the serum of 66 patients whose values for serum amylase were above normal. Three patients (4.5%) were identified by this method as having macroamylase , and this was confirmed by gel-filtration chromatography and electrophoresis. We find this to be the test of choice as a sc...

For more than 50 years, total serum amylase activity has been the biochemical test most commonly used to confirm the clinical diagnosis of acute pancreatitis. Serum amylase activity has been hoisted onto this pedestal by default, because until recently it has been the only reproducible and rapid diagnostic assay available in clinically emergent settings. As a result of our longstanding dependen...

Background and objectives For most of the application cereal materials, sample pretreatments are considered critical factors. Different (roasting amylase hydrolysis) prior to extraction were employed obtain oat protein (OP) from grains. The effect pretreatment on structure functional properties OPs was determined. Findings Analysis sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-...

-Different amylase enzymes were identified by analysis of digestive fluid and haemolymph in diapausing and nondiapausing strains of silkworm, Bombyx mori. The diapausing strain showed negligible digestive amylase activity at a pH range of 3-11, while the nondiapausing strain registered strikingly higher amylase activity at pH 9.2. Higher levels of undigested starch was found in the faecal matte...