Diabetes mellitus is a multifactorial metabolic disease characterized by post-prandial hyperglycemia (PPHG). α-amylase and α-glucosidase inhibitors aim to explore novel therapeutic agents. Herein we report the promises of Dioscorea bulbifera and its bioactive principle, diosgenin as novel α-amylase and α-glucosidase inhibitor. Among petroleum ether, ethyl acetate, methanol and 70% ethanol (v/v)...

Abstract Diabetes mellitus is a metabolic disease characterized by hyperglycemia resulting from either defect in insulin secretion, insulin action, or both. Postprandial hyperglycemia is a prime characteristic of diabetes mellitus and has been a focus in the therapy for diabetes. One of the therapeutic approaches which involve decreasing hyperglycemia aims at lowering blood glucose...

The side effects associated with the usage of synthetic antidiabetic drugs make it imperative to search for alternative drugs from medicinal plants. Therefore, this study was aimed at evaluating the α-amylase and α-glucosidase inhibitory potential of Calotropis procera leaf, as well as its possible mode of inhibiting these enzymes. Acetone, aqueous and ethanolic extracts of C. procera leaf was ...

Inhibition of α-amylase is an important strategy to control post-prandial hyperglycemia. The present study on Ruellia tuberosa, known as traditional anti-diabetic agent, is being provided in silico study to identify compounds inhibiting α-amylase in rat and human. Compounds were explored from PubChem database. Molecular docking was studied using the autodock4. The interactions were further visu...

Drying of seeds of Agropyron desertorum (Fisch. ex Link) Schult. did not result in breakdown of alpha-amylase nor impair the ability of seeds to resume its synthesis when moistened again. beta-Amylase activity did not change during 5 days of germination at a water potential of 0 atmosphere nor during 40 days of incubation at -40 atmospheres. Seeds synthesized alpha-amylase at 0, -20, and -40 at...

Gibberellic acid (GA) enhances the synthesis of alpha-amylase and ribonuclease in isolated aleurone layers and this process is inhibited by abscisin. Removal of gibberellic acid in mid-course of alpha-amylase production results in a slowing down of alpha-amylase synthesis, suggesting a continued requirement of GA for enzyme synthesis. This is paralleled by a continuous requirement for RNA synth...

Water soluble cranberry-based phytochemical combinations with oregano, rosemary, and Rhodiola rosea were evaluated for total phenolic content, related antioxidant activity and inhibition of diabetes management-related alpha -glucosidase, pancreatic alpha-amylase inhibition, and hypertension-related ACE-I inhibitory activities. Water extracts of oregano had 114.9 mg/g DW of phenolics which was h...

Both polyethylene glycol (PEG) and mannitol inhibit gibberellic acid-induced alpha-amylase production in barley aleurone layers. The effect of the osmotic solution is on enzyme synthesis rather than alpha-amylase secretion. The inhibition of alpha-amylase synthesis does not appear to be mediated via an indirect effect on respiration or protein synthesis. Rather it seems that the osmotic solutio...

Modulating α-amylase enzyme activity with bioactive peptides of protein hydrolysate is one of a viable strategy in the management of diabetes through the control of postprandial glucose level. In this study, four proteolytic enzymes (pepsin, neutrase, alcalase and trypsin) were used to investigate the in vitro α-amylase inhibition of dried laver seaweed (Porphyra species) protein hydrolysates. ...

Human pancreatic α-amylase (HPA) inhibitors offer an effective strategy to lower postprandial hyperglycemia via control of starch breakdown. Limonoids from Azadirachta indica known for their therapeutic potential were screened for pancreatic α-amylase inhibition, a known anti-diabetic target. Studies were carried out to reveal their mode of action so as to justify their hypoglycemic potential. ...