A technique was developed to study microcolony formation by silicone-immobilized bacteria on polycarbonate membrane filters under anaerobic conditions. A sudden shift to anaerobiosis was obtained by submerging the filters in medium which was depleted for oxygen by a pure culture of bacteria. The technique was used to demonstrate that preinduction of nitrate reductase under low-oxygen conditions...

Methylene blue is the most extensively used chemical indicator of anaerobiosis. Its use as such an indicator was reported early in the literature (Smith, Wilder Quarter Century Book, p. 187, 1893). Techniques such as hanging strips of methylene blue-impregnated gauze inside an anaerobic jar were described by van Reimsdijk (Ned. Tijdschr. Geneesk. 66:1423, 1922). Present-day microbiologists ofte...

No statistical differences were found in the recovery of group A streptococci from throat culture specimens after overnight incubation of blood agar plates in 5% CO2 compared with anaerobiosis. Anaerobic incubation required many more subcultures and resulted in considerably greater technical time and expense.

Kluyveromyces lactis is a yeast which cannot grow under strict anaerobiosis. To date, no factors responsible for oxygen sensing and oxygen-dependent regulation of metabolism have been identified. In this paper we present the identification of the glucose sensor Rag4 as a factor essential for oxygen-dependent regulation of the fermentative pathway.

Long-term anaerobiosis was studied in the priapulid Haljcryptus spinulosus and the bivalves Astarte borealis and Arctica island~ca, species with an extremely high resistance to lack of oxygen, from Kiel Bight. This shallow, brackish environment is frequently affected by extended periods of oxygen deficiency. Animals were experimentally subjected to anoxia for up to 60 d. Glycogen was the most i...

are placed at the bottom of the jar, one of the usual oxygen indicators (for example, alkali^6' glucose-methylene-blue mixture) placed aloflj? with the cultures, and the jar sealed. After jj to 12 hours, according to the size of the jar an" the amount and quality of iron filings, the colour of the indicator is discharged and co1*^ plete anaerobiosis obtained. Approximately " grammes of iron fil...

Freshwater turtles of the Trachemys and Chrysemys genera are champion facultative anaerobes able to survive for several months without oxygen during winter hibernation in cold water. They have been widely used as models to identify and understand the molecular mechanisms of natural anoxia tolerance and the molecular basis of the hypoxic/ischemic injuries that occur in oxygen-sensitive systems a...