Retrograde labelling of retinal ganglion cells with optic nerve transection often leads to degeneration of ganglion cells in prolonged experiments. Here we report that an intact optic nerve could uptake retrograde tracers applied onto the surface of the nerve, leading to high efficiency labelling of ganglion cells in the retina with long-term survival of cells. This method labelled a similar nu...

The electrosensory lobe (ELL) of mormyrid electric fish is the first stage in the central processing of sensory input from electroreceptors. The responses of cells in ELL to electrosensory input are strongly affected by corollary discharge signals associated with the motor command that drives the electric organ discharge (EOD). This study used intracellular recording and staining to describe th...

Unitary discharges were recorded from the inferior mesenteric ganglion of decerebrate dogs. Eighty-one units were identified as sympathetic postganglionic neurons innervating the colon and rectum by collision test performed by stimulation of the lumbar colonic nerve. Discharges of four units were enhanced simultaneously with an increased outflow of the renal nerve by pinching a toe. Thus, those...

glaucoma remains one of the major causes of blindness in today's world. the progressive field of stem cell proposes an exciting potential for discovering novel therapies. here, we report the development of an easy and high throughput method for differentiation of retinal ganglion cells (rgc) and bipolar cells from human adipose tissue-derived mesenchymal stem cells (hadscs) using pax6 (+5a) gen...

Rod signals in the mammalian retina are thought to reach ganglion cells over the circuit rod-->rod depolarizing bipolar cell-->AII amacrine cell-->cone bipolar cells-->ganglion cells. A possible alternative pathway involves gap junctions linking the rods and cones, the circuit being rod-->cone-->cone bipolar cells-->ganglion cells. It is not clear whether this second pathway indeed relays rod s...

BACKGROUND: Anal smears are increasingly being used as a screening test for anal squamous intraepithelial lesions (ASILs). This study was undertaken to assess the usefulness and limitations of anal smears in screening for ASILs. METHODS: The cytomorphological features of 200 consecutive anal smears collected in liquid medium from 198 patients were studied and findings were correlated with resul...

Spontaneous neural activity has been recorded in the auditory nerve of cats as early as 2 days postnatal (P2), yet individual auditory neurons do not respond to ambient sound levels <90-100 dB SPL until about P10. Significant refinement of the central projections from the spiral ganglion to the cochlear nucleus occurs during this neonatal period. This refinement may be dependent on peripheral s...

Whole-mounted human fetal retinae of gestational ages 14-40 weeks have been studied. These preparations clearly show the distribution of retinal ganglion cells or their precursors across the retina, and the pattern of the retinal vessels and vessel primordia. The ganglion cell layer is present at 14 weeks of gestation and distribution of cells in this layer (ganglion cell precursors) is at firs...

We report that the most common retinal ganglion cell type that remains after optic nerve transection is the M1 melanopsin ganglion cell. M1 ganglion cells are members of the intrinsically photosensitive retinal ganglion cell population that mediates non-image-forming vision, comprising ∼2.5% of all ganglion cells in the rat retina. In the present study, M1 ganglion cells comprised 1.7±1%, 28±14...

in the present study, eight specimens of sheep (>1 year) of both sexes were dissected to provide a comprehensive description of the weight, allocation and nerve branches of the cranial cervical ganglion. the cranial cervical ganglion was found beneath the mandibular salivary gland. it was located ventromedial to tympanic bulla and ventrally to atlantic fossa. in three out of eight animals it wa...