A reliable and reproducible protocol has been developed for high frequency plant regeneration from 4-5 mm long leaf base segments of 4 days old in vitro germinated seedlings of indica rice (Oryza sativa) cultivar Rasi. The effect of age of seedlings, position of leaf base segments and optimum concentration of 2,4-D on callus induction frequency was investigated with a future aim to use leaf bas...

An efficient method of somatic embryogenesis using exogenous polyamines through suspension culture protocol was developed from leaf derived callus of spine gourd (Momordica dioica Roxb. ex. Willd.). Embryogenic callus was originated from cut slices of leaf explants on MS solid medium supplemented with 4.4 μM 2,4-D with addition of polyamines (putrescine, spermidine and spermine). Putrescine at ...

The ontogenic developmental stages of saffron somatic embryogenesis have been studied and characterized using light and electron microscopy. The embryogenic callus underwent internal segmented divisions with the formation of globular embryos that were attached to the callus surface by a broad multicelular structure. Further development of the embryoids was characterized by the emergence of a sh...

to produce embryonic callus in four grapevine cultivars i.e. yaghouti, bidane sefid, shahroodi and flame seedless, ovary explants were collected at flower developmental stages iii and v and cultured in ms medium supplemented with various concentrations of 2,4-d and bap. for differentiation of somatic embryos, ms medium was supplemented with various concentrations of iaa and bap. results showed ...

Abstract The goal of this research was to investigate the ontogenic path followed by somatic embryo from oil palm ( Elaeis guineensis jacq.) with in vitro culture and characterize shoot explants. Explant were injected into a medium for induction calluses, histological analysis perfomed on embryogenic callus embryo. Embryogenic nodular structure still proliferate because cells are meristematic v...

Friable callus was initiated from shoot apices of Gloriosa superba L. on basal MS medium supplemented with 2, 4-D (4mg L(-1)) + Kn(5 mg L(-1)) + CH(10 mg L(-1)) + CW(20%). Subculture of callus on the same medium after 4-5 weeks showed induction of large number of somatic embryos, which was confirmed with histological studies. Development of embryoids in plantlet took place when the embryogenic ...

Callus induction, somatic embryogenesis and plant regeneration were initiated in selected five species of Turkish crocus using three diffrent explants (leaf, stem and corm) cultured on four different media (MS, GB5, LS and CHE). The highest frequencies of callus induction (100%) and shoot regeneration (70%, with 7.2 shoots/callus) were found in the crocus species Crocus oliveri ssp. Oliveri, us...