Binding of the class II PapG adhesin, found at the tip of filamentous pili on Escherichia coli, to the carbohydrate moiety of globoseries glycolipids in the human kidney is a key step in development of pyelonephritis, a severe form of urinary tract infection. An assay based on surface plasmon resonance for quantification of the binding of the class II PapG adhesin to oligosaccharides has been d...

The use of thioglycosides and other glycan derivatives with anomeric sulfur linkages is gaining increasing interest, both in synthesis and in various biological contexts. Herein, we demonstrate the occurrence and circumvention of anomerization during 1-S-glycosylation reactions, and present highly efficient and stereocontrolled syntheses of a series of photoprobe-thiosaccharide conjugates. Muta...

Fully atomistic molecular dynamics simulation studies of thermotropic bilayers were performed using a set of glycosides namely n-octyl-β-D-glucopyranoside (β-C8Glc), n-octyl-α-D-glucopyranoside (α-C8Glc), n-octyl-β-D-galactopyranoside (β-C8Gal), and n-octyl-α-D-galactopyranoside (α-C8Gal) to investigate the stereochemical relationship of the epimeric/anomeric quartet liner glycolipids with the ...

It has been shown that the acetamido methyl protons of N-acetyl-D-glucosamine undergo a chemical shift to higher fields in their proton magnetic resonance spectrum when the inhibitor is bound to lysozyme. The observed chemical shift in the presence of the enzyme is different for the CP and /3-anomeric forms of Z-acetamido-Z-deoxy-D-glucopyranose indicating either a difference in the afhnity of ...

4,6-O-Ethylidene-N-(2-hydroxybenzylidene)-D-glucopyranosylamine was synthesized and characterized using analytical, spectral and single-crystal X-ray diffraction methods. The anomeric nature of the saccharide moiety was proposed based on H NMR studies and was confirmed by the crystal structure. The lattice structure of this compound was compared with that of its analogues.

The subject paper presented the ®rst confor-mational analysis of the anomeric e€ect within the context of molecular orbital theory, discussed the utility of Fourier decomposition of a torsional coordinate as a method for analyzing disparate electronic in¯uences on that coordinate, and helped settle debate on the nature of anomeric stabilization. Sugars are the stepchildren of the biomonomer fam...

The carbohydrate structure of the BIN and F glycoproteins of the paramyxovirus SVS grown in bovine kidney (MDBK) cells has been elucidated by newly developed micromethods. The glycoproteins were purified by chromatography on fetuin coupled to Sepharose and on hydroxylapatite. Extensive aggregation of the F glycoprotein at concentrations > 0.1 mg/ml was reduced by carboxymethylation. The glycopo...

The major cellobiose dehydrogenase (oxidase) (CBDH) secreted by the soft-rot thermophilic fungus Humicola insolens during growth on cellulose has been isolated and purified. It was shown to be a haemoflavoprotein with a molecular weight of 92 kDa and a pI of 4.0, capable of oxidizing the anomeric carbon of cellobiose, soluble cellooligosaccharides, lactose, xylobiose and maltose. Possible elect...

The disaccharide unit of the glomerular basement membrane has been studied in regard to its detailed structure and its linkage to the peptide portion. Graded acid hydrolysis of glycopeptides containing this unit resulted in a more rapid release of glucose than galactose and indicated that glucose was in the external position. Isolation of a disaccharide consisting of glucose and galactose with ...