PURPOSE To report a series of 3 patients with soft contact lens-related Fusarium keratitis. Two of them were treated with the antiamoebic polyhexamethylene biguanide 0.02% (PHMB) in combination with antifungal drugs, and 1 patient was treated with PHMB as sole antifungal regimen. METHODS Chart review of 3 patients treated with PHMB in Fusarium keratitis. Two of them were refractory to the com...

Three methods are presented for the simultaneous determination of diloxanide furoate (DLX) and metronidazole (MTR), used for their antiprotozoal and antiamoebic effect, in the presence of DLX alkaline degradates and in pharmaceutical formulations, without previous separation. The first method is chemometric-assisted spectrophotometry, in which principal component regression and partial least sq...

PURPOSE To report the histopathological findings in a case of severe Acanthamoeba sclerokeratitis (ASK). PATIENT AND METHODS A 46-year-old patient was referred to the Department of Ophthalmology of Mie University Hospital because of a severe corneal ulcer of the right eye of 6 months' duration. Our initial examination showed a ring-shaped corneal opacity with extensive epithelial defects and ...

A 35 years old male presented with a two months history of progressively increasing dyspnoea. On examination he was in respiratory distress, afebrile, had prominent neck veins and an increased area of cardiac dullness. The heart sounds were muffled, and the abdomen was unremarkable except for an enlarged, tender liver. The blood count was normal, but the ESR was raised (60mm). X-ray chest showe...

BACKGROUND & OBJECTIVES Amoebiasis, caused by Entamoeba sp. a protozoan parasite, is a major public health problem in tropical and subtropical countries. The symptomatic patients are treated by specific chemotherapy. However, there are reports of treatment failure in some cases suggesting the possibility of drug resistance. The present study was therefore planned to assess the presence and expr...

OBJECTIVES To determine the mechanism of trifluoromethionine resistance in Entamoeba histolytica and evaluate the impact of acquired drug resistance on virulence. METHODS Trifluoromethionine-resistant amoebae were selected in vitro and examined for cross-resistance to antiamoebic drugs, stability of resistance, methionine γ-lyase (MGL) activity, cell adhesion and virulence. Targeted gene sile...

Due to long-term treatment toxicity and clinical resistance to drugs commonly used against E. histolytica, new drugs against amoebiasis are urgently needed. Castela texana ("chaparro amargo") is a shrub taken traditionally in teas and capsules of dry plant to treat intestinal amoebic infections. An aqueous extract was prepared and its mutagenic, genotoxic and cytotoxicity properties were evalua...

A total of 72 mice were used for an experimental model of intestinal amoebiasis developed in the Swiss albino mice dividing into three groups, experimental immunocompetent mice group (Group-A), experimental immunodepressed mice group (Group-B) and control group (Group-C). The inoculum (10 cells of Entamoeba histolytica/0.2 ml of medium) was used to inoculate into the caecum of the experimental ...

In Mexico, the Adenophyllum aurantium (L.) Strother plant is consumed as an infusion to treat intestinal diseases such as amoebiasis, which is an endemic health problem in Mexico and other countries. However, the effect of A. aurantium on Entamoeba histolytica, the causative agent of amoebiasis, is unknown. An aerial part methanolic extract (AaMeA), a root methanolic extract (AaMeR) and a root ...