............................................................................................................................................................................. 4 Introduction ........................................................................................................................................................................ 5 Methods.................................

Several distinct chromosomal segments were recently identified by cosegregation analysis of polymorphic markers with antibody responsiveness in an F2 cross between high (H) and low (L) antibody responder lines of Biozzi mice. The effect associated with the relevant markers has now been investigated in backcross populations (toward the L line) bred from H and L mice made coisogenic at the H-2 lo...

Previous studies with 31 strains of Serratia marcescens, including 28 reference O-serotype strains, have indicated that 19 of them have an acidic polysaccharide which copurifies with lipopolysaccharide during phenol-water extraction. Polysaccharide in crude extracts from 18 of the 19 strains was precipitated with Cetavlon (hexadecyltrimethyl ammonium bromide), and capsules were demonstrated aro...

A reverse-sandwich enzyme-linked immunosorbent assay (ELISA), in which an antibody is sandwiched by antigens, was established for the titration of antibodies to verocytotoxins (VT) in human and animal sera. This assay has two advantages over a conventional indirect ELISA: (i) higher specificity and sensitivity and (ii) the ability to comparably titrate antibodies from different species. The VT1...

The main aim of this study was to find a suitable rabies vaccination protocol which would allow usage of lower volumes of vaccine, fewer clinical visits and above all, enough serological effectiveness for high-risk individuals requiring pre-exposure immunization. Human diploid cell rabies vaccine was administered intradermally and intramuscularly in 60 previously unvaccinated volunteers, aged 1...

Herein, we report on the fabrication of an extended-gated organic field-effect transistor (OFET)-based immunosensor and its application in the detection of human chromogranin A (hCgA). The fabricated OFET device possesses an extended-gate electrode immobilized with an anti-CgA antibody. The titration results of hCgA showed that the electrical changes in the OFET characteristics corresponded to ...

the measurement of anti-streptokinase antibodies can distinguish the patients who may benefit from streptokinase and those who should be treated with some other thrombolytic regimen. neutralising titration test is a commonly used classical assay for measuring anti-streptokinase antibodies and in this assay the ability of anti-streptokinase antibodies in patients' sera in preventing the lytic ef...

Ross, Martin R. (Connecticut State Department of Health, Hartford) and Earle K. Borman. Direct and indirect fluorescent-antibody techniques for the psittacosis-lymphogranuloma venereum-trachoma group of agents. J. Bacteriol. 85:851-858. 1963.-Direct and indirect fluorescent-antibody (FA) techniques were developed for the detection of group antigen in infected tissue cultures and the titration o...

Potentiometric and spectrophotometric titrations of staphylococcal enterotoxin B have been carried out in 0.16 M KC1 at 25”. Potentiometric titration results indicate that the titration curves are essentially reversible between pH 1.50 and 11.0. All of the ionizable groups in enterotoxin B found from the titration curves are consistent with the amino acid composition which has been reported in ...

Introduction: The antibody titre offered by the Royal College of Pathologists Australasia Quality Assurance Programs (RCPAQAP) surveys laboratory technical procedures on titres using various methods. A review is performed reports last 5 years to ascertain any differences in sensitivity between methods employed laboratories. Method: Titre results were extrapolated from 20 samples over years. are...