and a loss of weight. The pooled sera contained 0.68 mg of antibody N per ml as determined by the quantitative precipitin test of Heidelberger and Kendall (J. Exptl. Med. 50:809, 1929). Standard curves for both the gel diffusion test and the precipitin test were prepared with the purified toxin and antitoxin. Agar diffusion plates were incubated at 30 C in a glass-walled water bath. The movemen...

PURPOSE Serodiagnosis of tuberculosis in children, using available crude antigens, has been difficult. The tests lack sufficient sensitivity and/or specificity. In this study, western blot analysis of M. tuberculosis H37Rv culture filtrate antigen (CFA) was carried out, to identify diagnostically useful antigens. In addition, the CFA was also used in enzyme linked immunosorbent assay (ELISA), t...

Indigenous actinomycetes isolated from rhizosphere soils were assessed for in vitro antagonism against Colletotrichum gloeosporioides and Sclerotium rolfsii. A potent antagonist against both plant pathogenic fungi, designated SRA14, was selected and identified as Streptomyces hygroscopicus. The strain SRA14 highly produced extracellular chitinase and beta-1,3-glucanase during the exponential an...

Diseases in rice plants (paddy) caused by microorganisms such as Pyricularia oryzae lead to a decrease production. Therefore, it is essential find out biological agents for protecting paddy and general, against plant diseases. Liquid smoke endophytic fungi have been known enhance the protection of disease. The purpose this study was determine ability liquid smoke, concentrations combinations su...

background:  aeromonas spp. can cause diarrhea and various infections in humans. access to rapid techniques with a high sensitivity and specificity is strongly needed for the identification of aeromonas species. the aim of this study was to evaluate two different methods including api 20e bacterial identification tests and the molecular detection using pcr primers specific for 16s-rrna and 23s-...

The cutaneous reaction demonstrates that the culture lysate of Streptococcus scarlatinae is approximately ten time more potent in its toxic effect than is the culture filtrate since repeated and carefully controlled human skin tests show that 0.1 cc. of a 1:2000 dilution of lysate reacts equally as well as a similar dose of a 1:250 dilution of culture filtrate (Dick's standard skin unit). Anima...

Application programming interface (API) management delivers the business centricity and business model that many service-oriented architecture (SOA) initiatives historically have lacked, and SOA delivers the experience and engineering discipline that drives good API design and provides robust integration to systems of record. API management and classical SOA governance are highly synergistic. A...