By rationally employing enzymes for signal amplification, an ultrasensitive aptasensor was developed and successfully tested in a system designed to detect lysozyme with a detection limit of 0.1 fM. This detection limit is nearly three orders of magnitude lower than those of any previously reported DNAzyme-based aptasensors.

A simple modular aptamer-based sensor (aptasensor) platform was prepared by combining the merits of the rapid and efficient preparation of a self-assembled monolayer of cucurbit[7]uril (CB[7] SAM) and the strong and specific binding affinity of CB[7] to ferrocenemethylammonium (FA), as an ultrastable supramolecular linker, to immobilize aptamers on CB[7] SAM.

We developed a facile one-step approach to synthesize DNA-templated Ag/Pt bimetallic nanoclusters (DNA-Ag/Pt NCs), which possess highly-efficient peroxidase-like catalytic activity. With this finding, an aptamer based sandwich-type strategy is employed to design a label-free colorimetric aptasensor for the protein detection with high sensitivity and selectivity.

A novel surface-enhanced Raman scattering (SERS) assay is demonstrated by developing a cross strand-displacement amplification (CSDA) method to enhance the SERS signals. Highly sensitive and selective detection of any single-stranded DNA is achieved and an aptasensor for proteins is constructed.

Specific nucleic acid aptamers using the microtiter plate based modified SELEX method against explosive trinitrotoluene are reported. Efficient partitioning of dsDNA was carried out using streptavidin labeled gold nanoprobes for the selection of specific aptamers. The selected binders having an affinity of ~10(-7) M were used in the newly developed electrochemical aptasensor, exhibiting a detec...

To remedy the problems caused by the introduction of an additional electron mediator and realize signal amplification, a new strategy has been presented to construct an electrochemical aptasensor for thrombin detection based on the cascade electrocatalysis of alkaline phosphatase (ALP) and Pt nanoparticle (PtNP)-functionalized ZnO nanoflowers.

We present a nanoplasmonic aptasensor for adenosine triphosphate (ATP) by using single gold nanoparticles (GNPs) as probes. The specific aptamer-ATP binding induced conformational change could modulate the surface-dependent self-catalytic growth of GNPs, which enabled the detection of ATP with ultra-sensitivity and selectivity.

The continuous outbreaks of foodborne diseases have drawn public attentions to food safety. Early screening of foodborne pathogens is crucial to prevent and control of foodborne diseases. In this study, a novel electrochemical aptasensor was developed for rapid and sensitive detection of E. coli O157:H7 using the coaxial capillary with immune magnetic nanoparticles (MNPs) for specific separatio...

A novel photoelectrochemical aptasensor for adenosine triphosphate (ATP) detection was developed by introducing inorganic−organic photoactive nanocomposites as sensitization agents to achieve significant signal amplification. Specifically, a TiO2/Au hybrid structure was first fabricated by decorating Au nanoparticles on the surface of TiO2 film, which then was used as the photoelectrochemical m...

In this work, a sensitive electrochemical aptasensor for the detection of thrombin (TB) is developed and demonstrated based on the co-catalysis of hemin/G-quadruplex, platinum nanoparticles (PtNPs) and flower-like MnO2 nanosphere functionalized multi-walled carbon nanotubes (MWCNT-MnO2).