tries. Most allergic reactions are mediated by allergen-specific IgE antibodies. Therefore, development of a method for inhibiting allergen-specific IgE production is a useful approach to prevent food-allergic diseases. IgE synthesis is considered to be caused by development and activation of T helper-2 (Th2) cells and B cells. This specific Th2 cell produces predominantly interleukin (IL)-4 an...

Amplified Fragment Length Polymorphism (AFLP) was used to establish the genetic relationships among 20 species from seven of the nine sections of genus Arachis. The level of polymorphism among nine accessions of the cultivated peanut, A. hypogaea L., was also evaluated. Three combinations of primers were used to amplify the AFLPs. The fragments were separated in 6% denaturing acrylamide gels. A...

A mapping population of recombinant inbred lines (RILs) derived from TMV 2 and its mutant, TMV 2-NLM was employed for mapping important taxonomic and productivity traits using genic and non-genic transposable element markers in peanut. Single nucleotide polymorphism and copy number variation using RAD-Sequencing data indicated very limited polymorphism between TMV 2 and TMV 2-NLM. But phenotypi...

The groundnut, Arachis hypogaea seedlings, when grown in pot cultures for 10-25 days at 25, 50, 100 microM CdCl2, showed a marked decline in growth compared to control. Similar trend was observed for nitrate reductase (NR) and nitrite reductase (NiR) activities whereas proline, peroxidase (POD) and catalase (CAT) showed increasing trend when observed on the 10th day of the experiment. Changes h...

Studies have demonstrated that nucleotide-binding site-leucine-rich repeat (NBS-LRR) genes respond to pathogen attack in plants. Characterization of NBS-LRR genes in peanut is not well documented. The newly released whole genome sequences of Arachis duranensis and Arachis ipaënsis have allowed a global analysis of this important gene family in peanut to be conducted. In this study, we identifie...

Rapid clonal propagation through in vitro techniques using cotyledonary node as an explant has been attempted in the high yielding, tikka-susceptible (ICG 11337, AK 1224, ICGS 44, JL 24) and tikka-immune (ICG 6284) varieties of Arachis hypogaea L. Cotyledonary nodes were excised from the 12–15 days-old axenic seedlings and cultured in the presence of various concentrations of N6 –benzylaminopur...

The ravages of the boll weevil have made it unprofitable to grow cotton in various sections throughout the South. In many regions where the growing of cotton has been decreased, peanuts are now being grown as a supplementary crop. This has caused a large increase in the production of peanuts in the United States. A number of mills which in the past produced cottonseed oil are now using their pr...

Data obtained by independent tests on each of 483 batches of Rhizobium inoculants for Glycine max, Medicago sativa, and Arachis hypogaea, manufactured commercially in South Africa, are reported and discussed. Whereas the average cell count per gram per batch was well in excess of 10, inoculants for G. max and M. sativa manufactured with peat treated with gamma irradiation at a dose of 50 kGr co...

In this study, a full-length cDNA of the acyl-ACP thioesterase, AhFatA, was cloned from developing seeds of Arachis hypogaea L. by 3'-RACE. Sequence analysis showed that the open reading frame encodes a peptide of 372 amino acids and has 50-70% identity with FatA from other plants. Real-time quantitative PCR analysis revealed that AhFatA was expressed in all tissues of A. hypogaea L., but most ...

The cultivated peanut (Arachis hypogaea L.) is an allotetraploid (AABB) species derived from the A-genome (Arachis duranensis) and B-genome (Arachis ipaensis) progenitors. Presence of two versions of a DNA sequence based on the two progenitor genomes poses a serious technical and analytical problem during single nucleotide polymorphism (SNP) marker identification and analysis. In this context, ...