Compartmentalization is a ubiquitous feature of life, with membrane interface that regulates molecular transport and exhibits bioactivities. An artificial enzyme-integrated cascade system can mimic such interactive properties across different length scales for in vitro application. Here, it shown reticulated nanoporous framework nano-caged enzymes presents the first modular macroscale platform ...

• Enzymes combined with artificial membranes in biorefinery promote process intensification. The use of MBRs permit enzymes re-use and increased stability. removal enzyme inhibitors continuous operation. MBR biofuels, phytotherapics food ingredients production was analyzed. In this review, for the first time, conjugation major types used biorefineries membrane processes to develop different con...

A challenge in chemistry is an artificial enzyme that mimics the functions of the natural system but is simpler than proteins. The intensive development of artificial enzymes that use a variety of matrices, the rapid progress in supramolecular gels, and the apparent “superactivity” exhibited by supramolecular hydrogel-immobilized enzymes, prompted us to evaluate whether supramolecular hydrogels...

We report the thermodynamically controlled growth of solution-processable and free-standing nanosheets via peptide assembly in two dimensions. By taking advantage of self-sorting between peptide β-strands and hydrocarbon chains, we have demonstrated the formation of Janus 2D structures with single-layer thickness, which enable a predetermined surface heterofunctionalization. A controlled 2D-to-...

CYP175A1 is a thermostable P450 Monooxygenase from Thermus thermophilus HB27, demonstrating in vivo activity towards beta-carotene. Activity of CYP175A1 was reconstituted in vitro using artificial electron transport proteins. First results were obtained in the mixture with a crude Escherichia coli cell extract at 37 degrees C. In this system, beta-carotene was hydroxylated to beta-cryptoxanthin...

Nanoscale particles are known to exhibit novel and unprecedented properties that make them quite different from their corresponding bulk-scale materials. The key differences are (i) an increased relative surface area and (ii) quantum size effects. Since the growth, catalytic activity and the various interactions with molecules (including biomolecules) occur at the surface of nanoparticles, a gi...

The artificial cell is a Canadian invention (Chang, Science, 1964). This principle is being actively investigated for use in cell and organ replacements. The earliest routine clinical use of artificial cells is in the form of coated activated charcoal for hemoperfusion for use in the removal of drugs, and toxins and waste in uremia and liver failure. Encapsulated cells are being studied for the...

Background: Enzymatic cascades in metabolic pathways are spatially organized in such a way as to facilitate the flow of substrates. The construction of artificial cellulase complexes that mimic natural multienzyme assemblies can potentially enhance the capacity for cellulose hydrolysis. In this study, an artificial cellulase complex was constructed by tethering three cellulases to a synthetic p...

Abstract Base-modification can occur throughout a transfer RNA molecule; however, elaboration is particularly prevalent at position 34 of the anticodon loop (the wobble position), where it functions to influence protein translation. Previously, we demonstrated that queuosine modification be substituted with an artificial analogue via queuine tRNA ribosyltransferase enzyme induce disease recover...

Abstract A novel and sustainable tandem‐catalysis system for asymmetric synthesis is disclosed, which fabricated by bio‐inspired self‐assembly of artificial arthropod exoskeletons (AAE s) or fungi cell walls ( AFCW containing two different types catalysts (enzyme metal nanoparticles). The heterogeneous integrated enzyme/metal nanoparticle AAE / systems, contain chitosan as the main structural c...