In order to better understand how to prevent enzymatic greenning, it is important to understand  kinetic properties of polyphenol oxidase.  we investigated the effect of SDS on the rate of catechol oxidation by small cherry tomato partial purified PPO. PPO activity increased with increasing SDS concentration. The most effective concentration of SDS was 0.8, 1 and 1.25 mM in according to pH and ...

Background: Soil salinity negatively impacts agricultural productivity. Consequently, strategies should be developed to inculcate a tolerance in crops for sustainable food production. Growth regulators play vital role regulating stress tolerance. Methods: Thus, we examined the effect of exogenous salicylic acid (SA) and alpha-tocopherol (TP) (100 mg/L) on morphophysio-biochemical responses two ...

Polyphenol oxidase (PPO) activity varied in differentiation from hyphae to rhizomorph and croute in A. mellea. In undifferentiated hyphae there was not any PPO activity, while croute showed the highest activity. Electrophoresis in the presence of SDS allows the detection of intensive enzyme forms of PPO, and can also convert slower migrating enzyme forms to faster ones. Some fluorescent co...

Chemical herbicides are the most common method of weed control in crops, but they can also negatively affect host such as wheat (Triticum aestivum L.). The damage caused to crop plants is often temporary and minor, sometimes, it be more substantial, requiring remedial measures. Salicylic acid (SA) a plant hormone widely used promote growth mitigate oxidative stress through its exogenous applica...

We studied the response of each ascorbate peroxidase (APX) isoenzyme in spinach leaves under stress conditions imposed by high light intensity, drought, salinity, and applications of methyl viologen and abscisic acid. The steady-state transcript level of cytosolic APX remarkably increased in response to high-light stress and methyl viologen treatment, but not in response to the other stress tre...

An experiment was conducted to investigate the effects of zinc application and salinity stress (0, 33, 66 and 99 mM NaCl) on some morphological and physiological parameters of soybean (Glycine max L., var. Williams). Results showed that zinc application improved shoot length, root fresh and dry weight and shoot fresh and dry weight under all salinity treatments. The catalase (CAT), ascorbate pe...

this study was conducted on two canola (brassica napus) cultivars, okapi a winter type and cold tolerant and rgs003 a spring type and cold sensitive. seedlings were grown in an environmentally controlled growth room with 16 h d-1 photoperiod at 22/16 °c (day/night, control). at the 4-leaf stage, half of pots were transferred to a cold growth room for 7 d at 10/3°c (day/night, cold treatment) an...

Banana blossoms are rich in fiber and nutrients a popular plant-based, vegan alternative to fish. However undesirable browning, usually visible at the peduncle cut-end, negatively impacts consumer acceptability of banana blossoms. The aim this work was develop safe alternatives prevent browning First, activities primary enzymes associated with tissue i.e., polyphenol oxidase (PPO), peroxidase (...

The effect of acidified calcium sulfate (ACS) on the quality of litchi ( Litchi chinensis Sonn. cv. 'Brewster') fruit after harvest was evaluated. ACS at 1.25% or higher concentrations significantly inhibited the activities of polyphenol oxidase and peroxidase in the pericarp during storage at both 5 and 10 degrees C. These treatments also effectively prevented browning and retained the red col...

in order to study the effect of salicylic acid on antioxidant enzymes activity such as peroxidase (pod), gayacol peroxidase (gpx) and poly phenol oxidase (ppo), chlorophyll a, b and carotenoid in coriander, this study was done as completely randomized whit three replication in biocenter of zabol university, 2014. salicylic acid was in four level (0, 1, 1.5 and 2 mm). in comparison with control,...