UNLABELLED Targeting amino acid metabolism has therapeutic implications for aggressive brain tumors. Asparagine is an amino acid that is synthesized by normal cells. However, some cancer cells lack asparagine synthetase (ASNS), the key enzyme for asparagine synthesis. Asparaginase (ASNase) contributes to eradication of acute leukemia by decreasing asparagine levels in serum and cerebrospinal fl...

The Oncologist 2005;10:238–239 www.TheOncologist.com Correspondence: David S. Goodsell, Ph.D., Associate Professor, The Scripps Research Institute, Department of Molecular Biology, 10550 North Torrey Pines Road, La Jolla, California 92037, USA. Telephone: 858-784-2839; Fax: 858-784-2860; e-mail: [email protected] Website: http://www.scripps.edu/pub/goodsell Received January 5, 2005; accepted...

Asparagine-requiring Jensen and Walker rat tumor cells and their asparagine-independent variants have been analyzed. The following results were obtained: (1) Both cell lines have very low levels of asparagine synthetase, and non-requiring revertants isolated from these lines have elevated levels of the enzyme. (2) No differences in chromosome number were detected between the parent Jensen line ...

Maize seedlings were subjected to concentrations of 0 and 100 mM of NaCl in Hoagland’s nutrient solution medium in plastic pots containing perlite. Two levels of asparagine (5 and 10 mM) and glycerol (20 and 40 mM) were sprayed onto the leaves of maize seedlings 10 days after germination. Saline stress caused considerable decline in total dry mass, chlorophyll content and relative water content...

High-performance liquid chromatography (HPLC) analysis was used for identification of two problematic ureides, asparagine and citrulline. We report here a technique that takes advantage of the predictable delay in retention time of the co-asparagine/citrulline peak to enable both qualitative and quantitative analysis of asparagine and citrulline using the Platinum EPS reverse-phase C18 column (...

Targeting amino acid metabolism has therapeutic implications for aggressive brain tumors. Asparagine is an amino acid that is synthesized by normal cells. However, some cancer cells lack asparagine synthetase (ASNS), the key enzyme for asparagine synthesis. Asparaginase (ASNase) contributes to eradication of acute leukemia by decreasing asparagine levels in serum and cerebrospinal fluid. Howeve...

Soybean plants can fix atmospheric N2 in the root nodule, a symbiotic organ with rhizobia. The primary forms of N transported from fixation are ureides, allantoate, and allantoin, supplemented asparagine. nitrate absorbed roots is to shoots NO3− asparagine little portion ureides. concentrations N-metabolites were analyzed by capillary electrophoresis after supplying various urea, precursors all...

When Jensen sarcoma cells were cultured in medium devoid of free L-asparagine, the cell population decreased exponen tially for 10 days. The surviving cells then began an exponen ti@alproliferation phase. The resulting population did not quire asparagine to maintain proliferation. Population growth curves and cloning studies showed that, per 106 original cells, approximately 102 variant cells w...