نتایج جستجو برای: atp content

تعداد نتایج: 473151  

Journal: :Human reproduction 2007
Hai-tao Zeng Zi Ren William S B Yeung Yi-min Shu Yan-wen Xu Guang-lun Zhuang Xiao-Yan Liang

BACKGROUND Birefrigent meiotic spindle in live human oocytes can be visualized by the PolScope. This study investigated the relationship between birefrigent meiotic spindle and cytoplasmic mitochondrial DNA (mtDNA) and ATP contents in in vitro matured human oocytes. METHODS Oocytes at germinal vesicle stage were collected and cultured for 24-48 h with or without the metabolic inhibitor, carbo...

Journal: :Journal of applied physiology 2007
Gary C Sieck Wen-Zhi Zhan Young-Soo Han Y S Prakash

Denervation (DNV) of rat diaphragm muscle (DIAm) decreases myosin heavy chain (MHC) content in fibers expressing MHC(2X) isoform but not in fibers expressing MHC(slow) and MHC(2A). Since MHC is the site of ATP hydrolysis during muscle contraction, we hypothesized that ATP consumption rate during maximum isometric activation (ATP(iso)) is reduced following unilateral DIAm DNV and that this effec...

Journal: :Journal of cell science 1995
G Perchec C Jeulin J Cosson F André R Billard

Carp spermatozoa are immotile in seminal plasma or in saline solution of high osmolality (> 400 mosmol kg-1). These 'quiescent' spermatozoa initiate a progressive forward motility when transferred in freshwater or in saline solution with low osmolality (< 160 mosmol kg-1). In this study we investigated 'in vitro' the relationship between sperm ATP content (measured by bioluminescence) and sperm...

Journal: :Applied and environmental microbiology 1978
M Reichgott L H Stevenson

Sediments from natural marsh and four microecosystem (MES) tanks were compared with reference to ATP, carbon content, and physical properties. Mean values for bacterial numbers and ATP were about twice as high in the MES, carbon content was slightly higher in the artificial systems, and the sediment of the MES was enriched for larger grain sizes and depleted of silt and clay. In the natural mar...

Journal: :American journal of physiology. Regulatory, integrative and comparative physiology 2003
B Drew S Phaneuf A Dirks C Selman R Gredilla A Lezza G Barja C Leeuwenburgh

Mitochondria are chronically exposed to reactive oxygen intermediates. As a result, various tissues, including skeletal muscle and heart, are characterized by an age-associated increase in reactive oxidant-induced mitochondrial DNA (mtDNA) damage. It has been postulated that these alterations may result in a decline in the content and rate of production of ATP, which may affect tissue function,...

Journal: :American journal of physiology. Regulatory, integrative and comparative physiology 2002
Hong Ji Grazyna Graczyk-Milbrandt Mary D Osbakken Mark I Friedman

The fructose analog 2,5-anhydro-D-mannitol (2,5-AM) stimulates feeding in rats by reducing ATP content in the liver. These behavioral and metabolic effects occur with rats fed a high-carbohydrate/low-fat (HC/LF) diet, but they are prevented or attenuated when the animals eat high-fat/low-carbohydrate (HF/LC) food. To examine the metabolic bases for this effect of diet, we assessed the actions o...

Journal: :Journal of orthopaedic research : official publication of the Orthopaedic Research Society 2011
Hanan N Fernando Jessica Czamanski Tai-Yi Yuan Weiyong Gu Abdi Salahadin Chun-Yuh Charles Huang

Research has shown that mechanical loading affects matrix biosynthesis of intervertebral disc (IVD) cells; however, the pathway(s) to this effect is currently unknown. Cellular matrix biosynthesis is an energy demanding process. The objective of this study was to investigate the effects of static and dynamic compressive loading on energy metabolism of IVD cells. Porcine annulus fibrosus (AF) an...

2008
Brigitte Pelzmann Peter Schaffer Petra Lang Seth Hallstroem Karl Nadlinger George D. Birkmayer Gilbert Reibnegger Bernd Koidl

The aim of this study was to investigate the effect of NADH-supplementation on the metabolic condition of isolated guinea pig ventricular cardiomyocytes using the pinacidil-primed IK(ATp) as an indicator of subsarcolemmal ATP concentration. Membrane currents were studied using the patch-clamp technique in the whole-cell recording mode at 36-37C. Under physiological conditions (4.3 mM ATP in the...

Journal: :Blood 1979
F H Kirkpatrick A G Muhs R K Kostuk C W Gabel

The densest 0.1%--1% of circulating red cells were separated from fresh blood, and the ATP content of a representative sample of such cells was determined. The dense ("old") cells had decreased amounts of ATP relative to unfractionated cells. However, the dense cells were also smaller, and the concentration of ATP in these cells was the same as in controls. Therefore, it seems unlikely that los...

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