The Northern Adriatic Sea is a key area of the Mediterranean Sea, strongly affected by freshwater inputs, mainly from Po River, which bring high amounts nutrients as well organic and inorganic particles. Free-living particle-attached prokaryotes were characterized 16S rRNA gene amplicon sequencing size-fractionated samples collected during diatom bloom in this area. diversity free-living prokar...

Dams represent the most significant anthropogenic disturbance to global rivers. Previous studies have shown that free-living and particle-attached microbes exhibited differentially in river reservoir ecosystems. However, little is known about dam’s effect on their co-occurrence patterns. Here, a random matrix theory (RMT)-based network approach was used construct microbial ecological networks f...

Many software engineers try very hard not to use the word “reuse” because many methods and tools intended to promote reuse have failed to meet expectations. As soon as they learn that a software product line involves reuse they turn away quickly. The “attached process” that accompanies every core asset in a product line makes software reuse effective instead of frustrating. In this issue of Str...

Cryptosporidium is a genus of waterborne protozoan parasites that causes significant gastrointestinal disease in humans. These parasites can accumulate in environmental biofilms and be subsequently released to contaminate water supplies. Natural microbial assemblages were collected each season from an eastern Pennsylvania stream and used to grow biofilms in laboratory microcosms in which influx...

In response to certain environmental signals, bacteria will differentiate from an independent free-living mode of growth and take up an interdependent surface-attached existence. These surface-attached microbial communities are known as biofilms. In flowing systems where nutrients are available, biofilms can develop into elaborate three-dimensional structures. The development of biofilm archite...

High-molecular-weight polycyclic aromatic hydrocarbons (HMW-PAHs) are natural components of fossil fuels that are carcinogenic and persistent in the environment, particularly in oil sands process-affected water (OSPW). Their hydrophobicity and tendency to adsorb to organic matter result in low bioavailability and high recalcitrance to degradation. Despite the importance of microbes for environm...

This study describes the integration of IWA's anaerobic digestion model (ADM1) within a computational model of microbial fuel cells (MFCs). Several populations of methanogenic and electroactive microorganisms coexist suspended in the anolyte and in the biofilm attached to the anode. A number of biological, chemical and electrochemical reactions occur in the bulk liquid, in the biofilm and at th...

Our previously published research was one of the pioneering studies on the use of metagenomics to directly compare taxonomic and metabolic properties of aquatic microorganisms from different filter size-fractions. We compared size-fractionated water samples representing free-living and particle-attached communities from four diverse habitats in the Columbia River coastal margin, analyzing 12 me...

Free-living and surface-associated microbial communities in sand-packed columns perfused with groundwater were compared by examination of compositional and functional characteristics. The composition of the microbial communities was assessed by bulk DNA extraction, PCR amplification of 16S ribosomal DNA fragments, separation of these fragments by denaturing gradient gel electrophoresis, and seq...

21 Resuspended sediment can increase plankton biomass and the growth of bacteria, thus 22 influence the coastal planktonic microbial food web. But little is known about resuspension 23 itself: is it a single massive change or a whole series of events and how does it affect the 24 quantity and quality of resuspended prokaryotic cells? We simulated the sequential erosion of 25 mud cores to better...