A red-shifted, mutated form of the jellyfish green fluorescent protein (GFP) under control of a TEF promoter was expressed at high levels in the filamentous fungus Aureobasidium pullulans. In the three transformants studied, all morphotypes of the fungus. including pigmented chlamydospores, expressed GFP and fluoresced brightly. Confocal microscopy showed that the intracellular distribution of ...

Saprophytic fungi are being increasingly recognized as etiologic agents of mycoses in immunosuppressed patients. We report a case of subcutaneous infiltration by Aureobasidium pullulans, likely due to traumatic inoculation, in a neutropenic patient during chemotherapy for chronic lymphocytic leukemia. The patient was treated with amphotericin B deoxycholate but was subsequently switched to itra...

The techniques of confocal microscopy and optical tweezers have shown themselves to be powerful tools in biological and medical research. We combine these methods to develop a minimally invasive instrument that is capable of making hydrodynamic measurements more rapidly than is possible with other devices. This result leads to the possibility of making scanning images of the viscosity distribut...

The role of microbial immigration in the veinal colonization pattern of Aureobasidium pullulans on the adaxial surface of apple leaves was investigated in two experiments at two periods (early and late seasons) in 2004 by applying green fluorescent protein (GFP)-tagged blastospores to the foliage of orchard trees. Individual leaves were resampled by a semidestructive method immediately after in...

We conducted a survey of fungal endophytes in 582 germinated seeds belonging to 11 Colombian cultivars of the common bean (Phaseolus vulgaris). The survey yielded 394 endophytic isolates belonging to 42 taxa, as identified by sequence analysis of the ribosomal DNA internal transcribed spacer (ITS) region. Aureobasidium pullulans was the dominant endophyte, isolated from 46.7 % of the samples. A...

Seeds of rice, jowar, ground nut and fingermillet were inoculated with microorganisms like Chaetomium globosum, Aureobasidium pullulans, Gliocladium roseum, Streptomyces sp. Pseudomonas pseudomallei YL-1 along with fertilizer (NPK 17:17:17) and were grown under laboratory conditions in earthen pots for forty five days. The phenotypic traits were analyzed and compared with control and fertilizer...

Fructosyltransferase enzymes was isolated and partially purified from Aureobasidium pullulans. The enzyme being intracellular was recovered from the fungal sp. by extraction method using citrate buffer (0.1 M; pH 5.5). Enzyme was Semipurified by salt fractionation method which results increase in specific activity of the enzyme. Study regarding kinetic behavior indicates that the enzyme exhibit...

A rolling-sphere viscometer (RSV) was constructed and evaluated for monitoring apparent viscosit~'es of fermentation broths in sha'ke flasks or test tubes. The apparatus was calibrated by measuring translational velocities of magnetic stainless ste.el spheres (0.16-0.95 em diameter) rolling in N~wto­ nian viscosity standards (50-12.500 mPa's) on surfaces inclined 20° to 70°. The method was appl...