In this study, the effects of commercially available entomopathogens, Nibortem (Verticillium lecani), Met52 (Metarhizium anisopliae), Nostalgist (Beauveria bassiana) and Priority (Paecilomyces fumosoroseus), a herbal insecticide, Nimbecidine (Azadirachtin), on pest aphids, Aphis fabae Acyrthosiphon pisum, were investigated. The corrected mortalities they caused to A. 78.41%, 87.50%, 65.91%, 63....

Using bioactivity-guided isolation of bioactive compounds; deacetylviguiestenin, 4-O-caffeoyl-2-methyloxirane-2-carboxylic acid and sandaracopimaradiene-1α,9α-diol were obtained from Tithonia diversifolia (Hemsl.) A. Gray leaves identified using spectroscopic methods. Their feeding deterrence activity against Sitophilus zeamais (Motsch.) including those methanol crude extract, fractions, the es...

Fruit flies have been a constant threat to agricultural commodities from the distant past and management of pest is very difficult. To reduce pesticide pressure slow down development resistance, different combinations insecticides biopesticides were evaluated against fruit flies. When rotated with in three spray schedule (applied at an interval 10 days) 12 for their efficacy as well economic vi...

The leaf and bark fraction extracts of Azadirachta indica A. Juss. (neem) grown in the foothills (subtropical region) of Nepal were evaluated for their antioxidant activity, total phenolic (TP) and total flavonid (TF) contents. HPLC method was employed to quantify the amount of azadirachtin and nimbin present in the seed, leaf and the bark extracts of neem. The result showed that the highest az...

Conventional methods to determine esterase activity from insects are composed of a three-step process where the enzyme is allowed to hydrolyze a 1-naphthyl acetate substrate, that reaction is quenched by a SDS detergent, and then a Fast Blue B dye complex is formed with 1-naphthol, the product of 1-naphthyl acetate hydrolysis. These methods measure dye-product complex rather than the product, 1...

BACKGROUND In order to increase our knowledge on the susceptibility of entomopathogenic nematode (EPN) species to agrochemicals, the compatibility of the infective juveniles (IJs) of the EPN (Steinernema and Heterorhabditis) with eight chemical and bio-insecticides was investigated under laboratory conditions. The effect of direct IJ exposure to insecticides for 6 and 24 h was tested in a Petri...