BACKGROUND GTPases of the immunity-associated protein family (GIMAPs) are predominantly expressed in mature lymphocytes. Studies of rodents deficient in GIMAP1, GIMAP4, or GIMAP5 have demonstrated that these GTPases regulate lymphocyte survival. In contrast to the other family members, GIMAP8 contains three potential GTP-binding domains (G-domains), a highly unusual feature suggesting a novel f...

Nodular lymphocyte predominant Hodgkin lymphoma appears in 5% of lymphoma. Because major biological and clinical differences with classical close relationship to T-cell/histiocyte-rich large B-cell lymphoma, lately the term nodular is accepted. The presence cells preserved phenotype a lack CD30 prerequisite for diagnosis Lymphocyte are typically embedded nodules B lymphocytes (growth patterns A...

CBA/N mice and F1 male mice, which are hemizygous for the CBA/N X chromosome, have an immune defect which is associated with the absence (deficiency) of a subpopulation of mature or late developing B lymphocytes. This characteristic was utilized to develop an antiserum that was specific for this subclass of B cells. C57BL/L mice were immunized with DBA/2 spleen calls, and the resulting antisera...

A substantial heterogeneity exists in the amount of immunoglobulin (Ig) on the surface of bone marrow-derived (B) lymphocytes as measured by labeling these cells with fluorescein-conjugated (F1) 1 anti-Ig and analyzing them by the technique of rapid flow microfluorometry (RFMF). Ig-bearing lymphocytes from neonatal mouse spleen and from adult mouse bone marrow are very diverse populations when ...

Transforming growth factor beta (TGF-b) is a mediator released by nearly all cell types. It has suppression activity on the immune system, but exactly how this effect is carried out is not clear. Previous experiments showed that IgG interacts with or carriers active TGF-b, that could suppresses cytotoxic T-cell responses to an immunogenic tumor in mice. Since T cell receptor (TCR) has structura...

Cells of the immune system are heterogeneous by function, origin, and physical properties and there is increasing interest in isolation and characterization of the various lymphocyte subsets. Previous studies in this laboratory have made use of a battery of physical separation methods (cell electrophoresis, adherence column fractionation, sedimentation velocity separation, and buoyant density s...