A study was carried out with a newly isolated bacterial strain yielding extracellular amylase. The phylogenetic tree constructed on the basis of 16S rDNA gene sequences revealed this strain as clustered with the closest members of Bacillus sp. and identified as Bacillus subtilis BI19. The effect of various fermentation conditions on amylase production through shake-flask culture was investigate...

To study the effect of inserted peptides on the secretion and processing of exported proteins in Bacillus subtilis and Escherichia coli, pBR322-derived DNA fragments coding for small peptides were inserted between the DNA coding for the 31 amino acid B. subtilis alpha-amylase signal peptide and that coding for the mature part of the extracellular thermostable alpha-amylase of B. stearothermophi...

Chemical modification of α-amylase from locale bacteria isolate Bacillus subtilis ITBCCB148 using citraconic anhydride has been performed. increase thermal stability of the enzyme has successfully been done. The results showed that the modified enzymes with citraconic anhydride with modification degree of 45, 73, 82, 83, and 88% have similar optimum pH of 5.5 and optimum temperature of 60°C; ki...

The impact of ultra violet radiations on alpha amylase producing ability of Bacillus subtilis (2620) was studied. Maximum enzymatic activity (crude) upto 17 folds were obtained from mutant strain exposed to 60 minutes ultra violet irradiation in comparison to parent strain. Purified enzyme showed molecular weight of 68 KDa and 54 KDa by SDS-PAGE in both parent and mutant strains. The SDS-PAGE o...

The aim of this study was production and partial purification of α-amylase enzyme by Bacillus licheniformis. B. Licheniformis was allowed to grow in broth culture for purpose of inducing α-amylase enzyme. Optimal conditions for amylase production by B. Licheniformis are incubation period of 120 h, temperature of 37 °C and pH 7.0. The α-amylase enzyme was purified by ion exchange chromatography ...

Microbial Alpha amylase is highly demanded industrial enzyme in various sectors such as pharmaceuticals, food, textile and detergents, etc. In this study optimized alpha amylase Production by a strain of Bacillus Subtilis MAFE 118079 in solid state fermentation (SSF) was initiated and Rice bran was used substrate for alpha amylase production. Linear multiple regression analysis showed that maxi...