Acetobacter xylinum sllbsp. sucrojlermentans BPR2001, a cellulose-producing bacterillm, that was newly isolated from a natural source, prodllced large amounts of the water-soluble po]ysaccharide, acetan. UDP-glucose is known to be the direct precllrsor in the synthetic pathways of both cellulose and acetan. We attempted to breed mutant strains and succeeded in obtalning olle, BPR3001A, which pr...

Bacterial cellulose (BC) is a suitable material for biomedical applications due to its remarkable proprieties, such us: biodegradability, biocompatibility and ready availability. In this paper the swelling and drug release profiles from bacterial cellulosecarboxymethyl cellulose (BC-CMC) composite hydrogels were investigated, in the aim to use such systems in controlled drug delivery field. Ibu...

Structural characteristics of microbial cellulose synthesized by two different methods have been compared using FT-IR and X-ray diffraction techniques. Cellulose synthesized by Acetobacter xylinum NQ-5 strain from agitated culture conditions is characterized by a lower Ia mass fraction than cellulose that was produced statically. Such a decrease was in good correlation with smaller crystallite ...

The "parallel-up" packing in cellulose Ialpha and Ibeta unit cells was experimentally demonstrated by a combination of direct-staining the reducing ends of cellulose chains and microdiffraction-tilting electron crystallographic analysis. Microdiffraction investigation of nascent bacterial cellulose microfibrils showed that the reducing end of the growing cellulose chains points away from the ba...

A powerful replica molding methodology to transfer on-demand functional topographies to the surface of bacterial cellulose nanofiber textures is presented. With this method, termed guided assembly-based biolithography (GAB), a surface-structured polydimethylsiloxane (PDMS) mold is introduced at the gas-liquid interface of an Acetobacter xylinum culture. Upon bacterial fermentation, the generate...

We report the ability of cellulose to support cells without the use of matrix ligands on the surface of the material, thus creating a two-component system for tissue engineering of cells and materials. Sheets of bacterial cellulose, grown from a culture medium containing Acetobacter organism were chemically modified with glycidyltrimethylammonium chloride or by oxidation with sodium hypochlorit...

A new screening method for inhibitors of cellulose biosynthesis is described. This method utilized three microbial strains; a cellulose-containing fungus Phytophthora, and a cellulose non-containing fungus Candida, and a bacterial strain of Acetobacter, a cellulose-producing acetic acid bacterium. The primary screen examined microbial cultures for selective growth inhibition against Phytophthor...

Cellulose is a biopolymer that has long been used as a biomaterial and its different sources, as Bacterial Cellulose (BC), have shown to possess impressive mechanical properties, which can lead to the development of new biomedical implants. Bacterial Cellulose is a polysaccharide secreted by bacteria as Gluconacetobacter xylinus: BC is composed of highly hydrated nanofibrils and it is character...

Recently, increasing attention has been given to bacterial cellulose-based membranes be applied as dressings for healing purposes. Bacterial cellulose (BC) is an attractive biomaterial due its unique structural characteristics such high porosity, water retention capacity, mechanical strength, low density, and biodegradability. One drawback of hydrogels that, after the first dehydration, capacit...

A bacterial strain isolated from the fermentation of Colombian homemade vinegar, Gluconacetobacter medellensis, was investigated as a new source of bacterial cellulose (BC). The BC produced from substrate media consisting of various carbon sources at different pH and incubation times was quantified. Hestrin-Schramm (HS) medium modified with glucose led to the highest BC yields followed by sucro...