For a long time it was thought that Bursaphelenchus xylophilus was the only agent of the pine wilt disease. Recently, it was discovered that there are bacteria associated with the nematodes that contribute to the pathogenesis of this disease, mainly through the release of toxins that promote the death of the pines. Among the species most commonly found, are bacteria belonging to the Bacillus, P...

BACKGROUND With long delays observed between sampling and availability of results, the usefulness of blood cultures in the context of emergency infectious diseases has recently been questioned. Among methods that allow quicker bacterial identification from growing colonies, matrix-assisted laser desorption ionisation time-of-flight (MALDI-TOF) mass spectrometry was demonstrated to accurately id...

background : an accurate and prompt diagnosis of bacterial arthritis is essential for earlier treatment and a good outcome. superantigens produced by staph. aureus are among the most lethal toxins. the paper objective was identification of common bacterial antigens and s.aureus superantigens in synovial fluid (sf) of children with negative culture and direct smear for other bacteria except for ...

Clinical diagnostics of chronic polymicrobial infections, such as those found in chronic wounds, represent a diagnostic challenge for both culture and molecular methods. In the current retrospective study, the results of aerobic bacterial cultures and culture-free bacterial identification using DNA analyses were compared. A total of 168 chronic wounds were studied. The majority of bacteria iden...

Staphylococcus aureus is one of the most important human pathogens, causing more than 500,000 infections in the United States each year. Traditional methods for bacterial culture and identification take several days, wasting precious time for patients who are suffering severe bacterial infections. Numerous nucleic acid-based detection methods have been introduced to address this deficiency; how...

Rapid and accurate identification of mastitis pathogens is important for disease control. Bacterial culture and isolate identification is considered the gold-standard in mastitis diagnosis but is time-consuming and results in many culture-negative samples. Identification of mastitis pathogens by PCR has been proposed as a fast and sensitive alternative to bacterial culture. The results of bacte...

OBJECTIVE To evaluate the performance of a veterinary urine dipstick paddle (UDP) for diagnosis and identification of urinary tract infection (UTI) in dogs and cats. DESIGN Prospective, randomized, blinded study. SAMPLE 207 urine specimens. PROCEDURES UDPs were inoculated by 2 investigators and incubated according to manufacturer's instructions. Results, including presence or absence of b...

  Background : An accurate and prompt diagnosis of bacterial arthritis is essential for earlier treatment and a good outcome. Superantigens produced by Staph. Aureus are among the most lethal toxins. The paper objective was Identification of common bacterial antigens and S.aureus superantigens in synovial fluid (SF) of children with negative culture and direct smear for other bacteria except fo...

BACKGROUND In Africa, there are several problems with the specific identification of bacteria. Recently, MALDI-TOF mass spectrometry has become a powerful tool for the routine microbial identification in many clinical laboratories. METHODOLOGY/PRINCIPAL FINDINGS This study was conducted using feces from 347 individuals (162 with diarrhea and 185 without diarrhea) sampled in health centers in ...

background: neisseria meninigitidis is one of the most frequently encountered microorganisms associated with central nervous system infections. the aim of this study was to evaluate a pcr-based assay for specific and rapid detection of n. meninigitidis in csf specimens.   methods : since april 2002 to july 2006, 130 csf specimens were collected from patients suspected of having bacterial mening...