A significant number of pathogenic microorganisms can be found in environmental reservoirs (air, water, soil). It is important to assess the viability status of these organisms to determine whether they pose a threat to public health. Classical methods for determining viability are time consuming. Hence, molecular methods have been developed to address this problem. Molecular methods offer spee...

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Recent biological terrorism events have indicated that bacterial spores such as Bacillus anthracis are real threat agents. Real time detection of biological agents is possible with the use of an ultraviolet Fluorescent Aerodynamic Particle Sizer (FLAPS) that measures particles' intrinsic fluorescence. It is important to know whether intrinsic fluorescence could be used to estimate agents' viabi...

Polymorphonuclear neutrophils (PMN) are the first cellular line of antibacterial host defense. They sense pathogens through recognition of pathogen-associated molecular patterns (PAMPs) by innate pattern recognition receptors, such as Toll-like receptors (TLR). The aim of this study was to investigate whether PMN sense bacterial viability and explore which viability factor could be involved in ...

Bacterial plasmolytic response to osmotic stress was evaluated as a physical indicator of membrane integrity and hence cellular viability. Digital image analysis and either low-magnification dark-field, high-magnification phase-contrast, or confocal laser microscopy, in conjunction with pulse application of a 1.5 M NaCl solution, were used as a rapid, growth-independent method for quantifying t...

Transports of bacteria in the atmosphere relate to climate and global hydrological cycles by acting as nuclei of ice-cloud formation, and affect the ecosystems and public health in the downwind ecosystems. Here we present quantitative investigations of airborne bacterial cells coupled with LIVE/DEAD BacLight assay in southwestern Japan to show that airborne bacteria were widespread with Asian d...

Part 1 issued November 1971 PAGE Effects of Organochlorine Insecticides on Bacterial Growth, Respiration and Viability. By P. W. TRUDGILL, R. WIDDUS and J. S. REES The Effects of Technical Chlordane on Energy Metabolism of Bacillus subtilis. By R. WIDDUS, P. W. TRUDGILL and M. J. MALISZEWSJSI The Effects of Technical Chlordane on Growth and Energy Metabolism of Streptococcus faecalis and Mycoba...

The effectiveness of SYTOX Green nucleic acid stain for measuring bacterial viability was tested on starved populations of Escherichia coli and Salmonella typhimurium. This stain underestimates the fraction of dead cells within starved populations containing cells with damaged nucleic acids or membranes. Its application to natural samples should be considered with caution.

A simple column filtration was developed to clarify meat homogenate suitable enough to quantify and assess the viability of bacterial population using flow cytometry. The removal of debris from homogenized meat suspensions was achieved by passing through different column materials such as sand particles, glass beads, dowex resin, and cell debris remover (CDR). Total plate counts on tryptic soy ...