We have developed a simple PCR assay protocol for detection of the root-knot nematode (RKN) species Meloidogyne arenaria, M. incognita, and M. javanica extracted from soil. Nematodes are extracted from soil using Baermann funnels and centrifugal flotation. The nematode-containing fraction is then digested with proteinase K, and a PCR assay is carried out with primers specific for this group of ...

The effects of vermicomposts on plant parasitic, fungivorous and baterivorous nematode populations were investigated in grape (Vitis vinifera) and strawberry (Fragaria ananasa) field crops. Commercially-produced vermicomposts derived from recycled paper, and supermarket food waste were applied to replicated plots at the rates of 2.5 t ha–1 or 5.0 t ha–1 for the grape crop and 5.0 t ha–1 or10 t ...

In a petri-dish study, development of the nematode Pratylenchus neglectus was observed every 4 days, and stage-specific development times were estimated, using a parameter estimation algorithm for a distributed-delay population model. The lower threshold temperature for development of a population of P. neglectus was 7.75 C. Temperatures above 25 C were unfavorable for this population on barley...

Reniform nematodes (Rotylenchulus spp.) have been reported to be associated with a large number of important products all over the world, ranging from cereals, vegetables and ornamental plants. In this study, morphologic molecular characters were used idetify Rotylenchulus population obtained soybean field in Adana province Turkey. Nematodes extracted soil using modified baermann funnel method....

background: panoramic imaging is one of the most common imaging methods in dentistry. regarding the side-effects of ionizing radiation, it is necessary to survey different aspects and details of panoramic imaging. in this study, we compared the absorbed x-ray dose around two panoramic x-ray units: pm 2002 cc proline (planmeca, helsinki, finland) and cranex tome (soredex, helsinki, finland). mat...

The objective of the study was to test for helminthes in sandboxes in day care centers in the city of Uberlândia, Southeastern Brazil in 2005. Fourteen private and 14 public day care centers with playgrounds were selected. One collection was carried out during the dry season (June and August), and the second one was in the rainy season (September and November). Samples were tested using Willis ...

The sugar-flotation-sieving (SFS) and Baermann-funnel (BF) methods were compared for nematode extraction efficiency. The SFS method recovered nematodes from more trophic groups whereas greater total numbers of individuals were recovered by BF. In a test to validate the efficiency of SFS, virtually 100% of the nematodes added to desert soil prior to extraction were recovered by four consecutive ...

Development of an in vitro culture system for infectious Dictyocaulus viviparus larvae made it possible to study the potential cross-transmission of D. viviparus between white-tailed deer (Odocoileus virginianus) and cattle (Bos taurus). Between 26 September 1995-29 February 1996, six parasite-free bull calves were individually inoculated with 15 to 50 infective third stage larvae (L3)/kg of bo...

Sensitive diagnostic tools are crucial for an accurate assessment of helminth infections in low-endemicity areas. We examined stool samples from Tanzanian individuals and compared the diagnostic accuracy of a real-time polymerase chain reaction (PCR) with the FLOTAC technique and the Kato-Katz method for hookworm and the Baermann method for Strongyloides stercoralis detection. Only FLOTAC had a...

Abstract Background Involvement of lacrimal apparatus by Non Hodgkin lymphoma as a first manifestation is extremely rare and only three cases have been reported in children. The purpose of this report is to determine the clinical characteristic of patient with primary involvement of lacrimal apparatus with lymphoma. Case presentation A 7-year-old girl with history of tearing, gradually prog...