Aerobically grown bakers’ yeast has been shown to contain two different lactic cytochrome c reductases, one of which is specific for L( +)-lactate (1) and the other of which is specific for D( -)-lactate (2). Neither of these two enzymes is present in anaerobically grown yeast, which contains instead a D( -)lactic dehydrogenase that does not reduce cytochrome c. No L( +)-lactic dehydrogenase is...

The biological treatment of wastewater discharged from a biodiesel fuel (BDF) production plant conducting alkali catalysis transesterification was investigated. BDF wastewater has a high pH and high hexane-extracted oil and low nitrogen concentrations, and inhibits the growth of microorganisms. The biological treatment of BDF wastewater is difficult because the composition of such wastewater is...

Enol esters of 2-substituted-3-oxoalkanoates of (Z)-configuration were reduced by bakers' yeast to chiral 2-substituted-3-hydroxyalkanoates. The sy/f-selectivities of this reaction increased compared with those of the reduction of the corresponding racemic keto esters. The reaction mayproceed via an asymmetric hydrolysis of the enol esters, followed by the reduction of the resulting carbonyl gr...

Jeener and Brachet (1) found that the absorption of inorganic phosphate by bakers’ yeast is greatly enhanced when the incubation of the yeast in a phosphate-containing nutrient (period of P absorption) is preceded by a period of incubation in a medium devoid of phosphate (period of P starvation). The Belgian authors had already demonstrated that a large part of the absorbed phosphate appears in...

Catalase from bakers’ yeast has been purified to homogeneity in the analytical ultracentrifuge and in gel electrophoresis; sedimentation measurements permit an estimation of its molecular weight as 248,000. Under denaturing conditions, polyacrylamide gel electrophoresis revealed dissociation of a major component of molecular weight 61,000, which constituted 90% of the total protein of the stain...