The influence of locked nucleic acid (LNA) residues on the thermodynamic properties of 2'-O-methyl RNA/RNA heteroduplexes is reported. Optical melting studies indicate that LNA incorporated into an otherwise 2'-O-methyl RNA oligonucleotide usually, but not always, enhances the stabilities of complementary duplexes formed with RNA. Several trends are apparent, including: (i) a 3' terminal U LNA ...

The successful development of high-affinity gapmer antisense oligonucleotide (ASO) therapeutics containing locked nucleic acid (LNA) or constrained ethyl (cEt) substitutions has been hampered by the risk of hepatotoxicity. Here, we present an in vitro approach using transfected mouse fibroblasts to predict the potential hepatic liabilities of LNA-modified ASOs (LNA-ASOs), validated by assessing...

This paper exhibits an examination, configuration, design to measure nonlinear characteristics of low noise amplifier(LNA) furthermore investigation, assess those estimations in the AWR microwave office Tool. A large portion of the critical aspects of LNA will be in linear measurements and which is composed, designed and simulated for the ultra LNA from 3GHz to 10GHz. In this proposed work the ...

We quantified incorporation rates of plasma-derived alpha-linolenic acid (alpha-LNA, 18:3n-3) into "stable" liver lipids and the conversion rate of alpha-LNA to docosahexaenoic acid (DHA, 22:6n-3) in male rats fed, after weaning, an n-3 PUFA-adequate diet (4.6% alpha-LNA, no DHA) or an n-3 PUFA-deficient diet (0.2% alpha-LNA, no DHA) for 15 weeks. Unanesthetized rats were infused intravenously ...

To study the hemodynamic and metabolic effects of chronic inhibition of endothelium-derived nitric oxide, we treated conscious rats with an oral solution of N omega-nitro-L-arginine (LNA), an inhibitor of nitric oxide production by endothelial cells. After 3 days of treatment with 2.74 mM LNA, rats had higher blood pressures (136 +/- 5 versus 113 +/- 3 mm Hg, p < 0.0005) than did the control an...

The simultaneous extraction of host plant DNA severely limits investigations of the community structures of plant-associated fungi due to the similar homologies of sequences in primer-annealing positions between fungi and host plants. Although fungal-specific primers have been designed, plant DNA continues to be excessively amplified by PCR, resulting in the underestimation of community structu...

An Ultra Wide Band CMOS Low Noise Amplifier (LNA) design is presented in this paper. Due to low power consumption and extremely high data rates the UWB system is bound to be popular in the end user market. The LNA is the first stage after antenna in an UWB transceiver. The LNA is accountable for providing enough gain to the signal with the bare minimum distortion. In this work we have designed ...

A design methodology for wideband CMOS low noise amplifier (LNA) with source degeneration is presented. By allowing an arbitrary source degeneration and employing a general input matching network, the minimum noise figure of the proposed wideband CMOS LNA can be made independent of the transistor width by properly choosing the source degeneration reactance. This result shows that the proposed t...

A highly linear, low voltage, low power, low noise amplifier (LNA) using a novel nonlinearity cancellation technique is presented in this paper. Parallel Inductor (PI) matching is used to increase LNA gain by 3 dB at the desired frequency. The linear LNA was designed and simulated in a TSMC 0.18μm CMOS process at 5GHz frequency. By employing the proposed technique, the IIP3 is improved by 12 dB...

Locked Nucleic Acid (LNA) is a unique nucleic-acid modification possessing very high binding affinity and excellent specificity toward complementary RNA or DNA oligonucleotides. The remarkable properties exhibited by LNA oligonucleotides have been employed in different nucleic acid-based therapeutic strategies both in vitro and in vivo. Herein, we highlight the applications of LNA nucleotides f...