The aim of the present study was to evaluate the protective effect of L-glutamine (L-Gln) against cryopreservation injuries on boar sperm. In Experiment 1, L-Gln from 20 to 80 mM was evaluated as a supplement for a standard freezing extender (egg yolk - EY - 20%, and glycerol 3%). No significant improvement (P>0.05) was obtained for any post-thaw sperm parameter assessed (objective sperm motili...

In modern livestock breeding, cryopreserved semen is routinely used for artificial insemination. Sperm cryopreservation secures future reproduction, and insemination doses can be easily shipped. Processing of semen for cryopreservation can be done with minimal efforts and relatively low costs. In this chapter we describe the entire cryopreservation process for stallion and bull sperm including ...

Oxidative stress during freezing-thawing reduces sperm motility, viability, membrane functions and antioxidant capacity. Thymus vulgaris has antioxidant properties due to phenolic compounds. The purpose of current study was to evaluate the effect of Thymus vulgaris extract as a natural antioxidant on post-thawed Moghani ram sperm quality. Semen samples were collected twice a week from four rams...

ABSTRACT: The present study evaluated the cryoprotectant efficacy of dimethylacetamide (DMA) and ethylene glycol in a one-step protocol to freeze boar sperm. sperm-rich portion ejaculates from two boars were collected once week, for 10 weeks. After collection, diluted (1:1; v/v) cooling extender. determining their spermatozoa concentration, pooled with same number each stabilized at 20°C 120 mi...

This study was conducted to examine if L-carnitine supplementation in the Tris-egg-yolk extender can improve the motility characteristics, membrane integrity, and in vitro fertilization potential after cryopreservation of ejaculated bovine spermatozoa. It was carried out on January to May 2013 at the University of Wisconsin-Madison. L-carnitine at final concentrations of 0.5, 1, 10, and 30mM we...

Background: Low density lipoprotein (LDL) of egg yolk has been successfully used for stallion and bull semen cryopreservation. However, there is limited information to clarify the effect of LDL or lyophilized LDL on ram semen quality. On the other hand, during semen collection, dilution and cryopreservation we need to protect the spermatozoa from lipid peroxidation. Clove bud has polyphenolic c...

Two experiments were designed to evaluate the effects of the amino acids glycine and cysteine on cryopreservation of ram spermatozoa. After primary evaluation of collected ejaculates, the semen samples were pooled and diluted 1:4 before cooling (experiment 1) and freezing (experiment 2) with Tris-Citrate-Fructose-Yolk (TCFY) extender supplemented with different concentrations of glycine and cys...

The aim of this study was to determine the effect of two variants of Tris-citric acid-fructose (TCF) extender containing whole hen egg yolk (TCF-HEY) and lyophilized lipoprotein fractions extracted from ostrich egg yolk (TCF-LPF(o)) on selected biological properties of cryopreserved sperm cells. Post-thaw percentage of motile sperm (MOT) was significantly higher (P < 0.05) for TCF-HEY extender ...

It is known that livestock animal semen very sensitive to cold shock during freezing processes, and this sensitivity directly affects post-thaw progressive motility, mitochondrial membrane potential, sperm nuclear DNA integrity in vitro spermatological parameters such as plasma acrosome integrity, fertility. In addition, with the sudden decrease total antioxidant level of after thawing, cells a...