Incorporation of noncanonical amino acids (ncAAs) can endow proteins with novel functionalities, such as crosslinking or fluorescence. In ion channels, the function these variants be studied great precision using standard electrophysiology, but this approach is typically labor intensive and low throughput. Here, we establish a high-throughput protocol to conduct functional pharmacological inves...

The microstructure of forged products significantly impacts their properties, and defects or carbide distribution are not visible to the naked eye. Isothermal compression tests on M50 steel with a Gleeble 3500 tester were conducted study behavior during forging. Tests examined hot deformation within temperature range 900–1200 °C strain rate 0.01–10 s?1. Power dissipation efficiency (?) flow ins...

Accumulating research suggests that heparanase may be a universal tumor-associated antigen (TAA). Several heparanase T-cell epitopes from humans and mice have already been identified. However, because of low immunogenicity, polypeptide vaccines usually have difficulty inducing effective antitumor immune responses in vivo. In this study, to increase the immunogenicity of polypeptide vaccines, we...

background: appropriate endurance exercise can change bone mass density and strength of weight-bearing bones in both animals and humans, but its effects on the microstructure are not known. this study aimed to determine skeletal changes induced by the treadmill running endurance in ovariectomized adult mice. methods: twenty-seven adult female rats were divided into experimental and control grou...

The paper expands the work by Kinnear and Weston[1] on squeeze film bearing design to the general case and compares the revised methodology with classical bearing design[2,3,4] and hybrid bearing design[5]. The analysis, which employs a partial differentiation of the Poisseuille and Hagen-Poiseuille bearing flow equations, enables a mathematical model for the bearing squeeze film to be develope...

A theoretical investigation into the prediction and measurement of squeeze film stiffness and damping for double hole entry hybrid journal bearings has been undertaken together with a review of previous work to identify critical bearing parameters. The analysis used a partial differentiation of the Poiseuille and Hagen-Poiseuille flow equations to develop a mathematical model for the bearing sq...

Simultaneous localization and mapping (SLAM) is an important research area in robotics. Lately, systems that use a single bearing-only sensors have received significant attention and the use of visual sensors have been strongly advocated. In this paper, we present a framework for 3D bearing only SLAM using a single camera. We concentrate on image feature selection in order to achieve precise lo...

The current study proposed a topology optimization method accounting for base material degradation and create a degradable device that retains sufficient stiffness through the degradation process to provide load bearings for tissue regeneration in orthopaedic applications. Degradable materials are less stiff than permanent materials and suffer further stiffness reduction through time when consi...

A sonar system is presented that relies on scanning a single ultrasonic transducer and measuring echo amplitude and arrival times. Bearing angles to targets are estimated far more accurately than the transducer beamwidth as obtained with conventional sonar rings based on the Polaroid ranging module. A Gaussian beam characteristic is fitted using least squares to the amplitudes of corresponding ...