Directed evolution approaches were used to construct a thermally stabilized variant of Erwinia chrysanthemi pectin methylesterase A. The final evolved enzyme has four amino acid substitutions that together confer a T(m) value that is approximately 11 degrees C greater than that of the wild-type enzyme, while maintaining near-wild-type kinetic properties. The specific activity, with saturating s...

Feruloyl esterases, a subclass of the carboxylic acid esterases (EC 3.1.1.1), are able to hydrolyse the ester bond between the hydroxycinnamic acids and sugars present in the plant cell wall. The enzymes have been classified as type A or type B, based on their substrate specificity for aromatic moieties. We show that Neurospora crassa has the ability to produce multiple ferulic acid esterase ac...

The isolation of component sugars from biomass represents an important step in the bioprocessing of sustainable feedstocks such as sugar beet pulp. Centrifugal partition chromatography (CPC) is used here, as an alternative to multiple resin chromatography steps, to fractionate component monosaccharides from crude hydrolysed sugar beet pulp pectin. CPC separation of samples, prepared in the stat...

INTRODUCTION 152 GUIDE TO TYPICAL FEEDSTUFFS 153 Cereals, sugar beet and oil seeds as feedstuffs 154 Major cereals fed to horses 154 Oil seeds 156 Vegetable oils 156 Forages and other fiber sources 158 Hay 158 Soaking hay before feeding 159 Semi-wilted forages 159 Silage 159 Straw 160 Hydroponics 160 Soya hulls and sugar beet pulp 161 Methods of feed processing 161 Feed storage 162 Feedstuffs a...

The objective of the present work was to relate the physical evolution quantified by image analysis to the chemical transformation of beet pulp particles during enzymatic degradation. Beet pulps were degraded into a torus reactor equipped for visualisation. Pectinolytic and cellulolytic enzymes were used separately or in combination. Two global image analysis techniques were tested to character...

The present experiment aimed at increasing orange peel and sugar beet pulp protein content through solid-state fermentation by Trichoderma reesei and Trichoderma viride. In vitro digestibility and changes in the chemical composition of the fermented products were determined after seven days of fungal cultivation using gas production tests. The cultivation of T. reesei and T. viride on orange pe...

Six multiparous, ruminally cannulated Holstein cows (46 DIM, 584 kg of BW) and 6 primiparous Holstein cows (35 DIM, 506 kg of BW) were used in two 6 x 6 Latin squares with 21-d periods to examine the effects of level of non-fiber carbohydrate, source of fibrous carbohydrate, and Aspergillus oryzae fermentation extract on intake, digestion, and milk production. Treatments were 42 and 36% non-fib...

White rot fungus Dichomitus squalens is an efficient lignocellulose degrading basidiomycete and a promising source for new plant cell wall polysaccharides depolymerizing enzymes. In this work, we focused on cellobiohydrolases (CBHs) of D. squalens. The native CBHI fraction of the fungus, consisting three isoenzymes, was purified and it maintained the activity for 60 min at 50°C, and was stable ...

The extraction of pectins from sugar beet pulp was carried out in an aqueous acid medium under different conditions using a full twostate experimental design for three extraction parameters (pH, temperature and time). The yields of the extracted pectins ranged from 4.1% to 16.2%. Their contents in pectin constituents were 35.2–76.3% galacturonic acid, 6.8–32.9% neutral sugars, 2.0–4.2% methoxy ...