The effect of pH on steady state kinetic parameters for the yeast alcohol dehydrogenase-catalyzed reduction of aldehydes and oxidation of alcohols has been studied. The oxidation of p-CH3 benzyl alcohol-l, l-h2 and -l,l-dz by NAD+ was found to be characterized by large deuterium isotope effects (&J&n = 4.1 f 0.1) between pH 7.5 and 9.5, indicating a rate-limiting hydride transfer step in this p...

The uptake and metabolism of the cytokinin benzyl adenine (BA) was compared in two lines of Petunia hybrida Vilm. differing in their shoot organogenic response. Leaf transfer experiments using shoot induction medium containing 4.4 micromolar BA showed that leaf explants from petunia line St40 required a shoot induction period of 6 to 10 days for commitment to shoot organogenesis; whereas leaf e...

the effect of sub culturing frequencies and different cytokinins (benzyl amino purine (bap), 2-isopentenyl adenine (2ip) on genetic stability of micropropagated shoots from olive plant were investigated by using physiological traits and random amplified polymorphic dna (rapd) markers. axillary buds of the olive (cv. dezful) plants were cultured and subcultured in dkw medium, supplemented with b...

Lignin is believed to be synthesized by oxidative coupling of 4-hydroxyphenylpropanoids. In native lignin there are some types of reduced structures that cannot be explained solely by oxidative coupling. In the present work we showed via biomimetic model experiments that nicotinamide adenine dinucleotide (NADH), in an uncatalyzed process, reduced a beta-aryl ether quinone methide to its benzyl ...

The effect of pH on steady state kinetic parameters for the yeast alcohol dehydrogenase-catalyzed reduction of aldehydes and oxidation of alcohols has been studied. The oxidation of p-CH3 benzyl alcohol-1,1-h2 and -1,1-d2 by NAD+ was found to be characterized by large deuterium isotope effects (kH/kD = 4.1 plus or minus 0.1) between pH 7.5 and 9.5, indicating a rate-limiting hydride trahsfer st...

An efficient, simple micro propagation method was developed for Zingiber officinale Rosc.) cv. Volvo using fresh sprouting bud in basal culture media. Explants were cultured on to Murashige and Skoog’s (MS) medium supplemented with different concentrations combinations of BA (Benzyl adenine) KIN (Kinetin) shoot IBA (Indol-3-Butyric acid) NAA (α- Naphthalene acetic root induction. MS 2.0 mg/L + ...

Cyclin-dependent kinases (cdk) control the cell division cycle (cdc). These kinases and their regulators are frequently deregulated in human tumours. A potent inhibitor of cdks, roscovitine [2-(1-ethyl-2-hydroxyethylamino)-6-benzylamino-9-isopropylpurin e], was identified by screening a series of C2,N6,N9-substituted adenines on purified cdc2/cyclin B. Roscovitine displays high efficiency and h...

Adenine together with certain 9-N-substituted derivatives such as 9-methyl, 9-benzyl, 9-benzhydryl, and 9-trityl were tested against Salmonella typhimurium strains TA97, TA98, and TA100 in the absence and presence of rat hepatic S9 prepared from Aroclor 1254 pretreated rats. All compounds were positive toward TA98 in the presence of the metabolic activating system, whereas they all lacked mutag...

The effect of pH on steady state kinetic parameters for the yeast alcohol dehydrogenase-catalyzed reduction of aldehydes and oxidation of alcohols has been studied. The oxidation of p-CH3 benzyl alcohol-l, l-h2 and -l,l-dz by NAD+ was found to be characterized by large deuterium isotope effects (&J&n = 4.1 f 0.1) between pH 7.5 and 9.5, indicating a rate-limiting hydride transfer step in this p...