The Brouwer-Heyting-Kolmogorov interpretation explains the meaning of logical operations as operators that construct proofs from proofs of the operands. The BHK interpretation is usually understood as giving intuitionistic interpretation for the logical operators, but, as pointed out by Troelstra and van Dalen [12], it is possible to understand the BHK interpretation classically. We elaborate t...

The amino acid sequence RGD (arginine-glycine-aspartic acid) is highly conserved in the VP1 protein of foot-and-mouth disease virus (FMDV), despite being situated in the immunodominant hypervariable region between amino acids 135 and 160. RGD-containing proteins are known to be important in promoting cell attachment in several different systems, and we report here that synthetic peptides contai...

The aim of this study is to investigate the effects of taurine treatment on cell viability and proliferations in BHK-21 cells. In this study, BHK-21 cells were seeded at a density of 50.000/ml per well in 96 well plates in medium composed of Glasgow-Minimum Essential Medium (GMEM). The cells were either left untreated (control) or treated with different concentrations of taurine for 24 h. XTT c...

Procedures are described for the purification of the Sindbis virus structural proteins. The amino acid and carbohydrate compositions of the purified proteins are presented for virus grown in BHK-21/13 and chicken embryo cells. Glycoprotein E1 from virus grown in BHK cells is deficient in a mannose-rich glycopeptide found on that glycoprotein when virus is grown in chicken embryo cells. The comp...

MATERIALS AND METHODS Virus Two strains of rubella virus were used, Judith (McCarthy & Taylor-Robinson, 1965) and Thomas, the latter having been isolated at St Thomas's Hospital in 1966 from an infant with congenital rubella. Judith was propagated in both RK 13 and BHK-21 cell cultures. Since its original isolation, Thomas had been passed eight times in RK 13 and BHK-21 cell cultures. Titres of...

rabies is acutely fatal encephalitis caused by a neurotropic virus. this virus belongs to the family ofrhabdoviridae and genus of lyssavirus. the virus is almost always transmitted to human through infectedmammalian saliva. rabies is inoculated to a wound by an infected animal bite. since infection is establishedin the cns, the outcome is almost always fatal. according to the outstanding role o...

A mutant line of BHK21 cells lacking inosinic pyrophosphorylase activity (IPP~ cells) does not incorporate [*H]hypoxanthine, as opposed to wild type BHK cells. When IPP+ and IPP~ cells are grown together in the presence of [H]hypoxanthine, IPP~ cells in contact with IPP+ cells incorporate the marker, as seen by autoradiography. This phenomenon is known as 'metabolic co-operation'. In the presen...

Baby-hamster kidney (BHK) cells were grown continuously in long-term monolayer culture in the presence of Swainsonine, an inhibitor of alpha-mannosidase II, a processing enzyme involved in glycoprotein biosynthesis. The asparagine-linked oligosaccharides (N-glycans) were isolated from Pronase-digested cells by gel filtration, ion-exchange chromatography and affinity chromatography on concanaval...

Evidence for a mechanism of initiation of viral persistence in which the cell, and not the virus, plays a critical role has been obtained using the important animal pathogen foot-and-mouth disease virus (FMDV). We have developed a virulence assay consisting of quantification of the ability of virus to kill cells and of cells to divide in the presence of virus and to initiate a carrier state. Ce...

Gammaretrovirus receptors have been suggested to contain the necessary determinants to mediate virus binding and entry. Here, we show that murine NIH 3T3 and baby hamster kidney (BHK) cells overexpressing receptors for subgroup A, B, and C feline leukemia viruses (FeLVs) are weakly susceptible (10(1) to 10(2) CFU/ml) to FeLV pseudotype viruses containing murine leukemia virus (MLV) core (Gag-Po...