نتایج جستجو برای: binding assays

تعداد نتایج: 511049  

Journal: :Pharmacological research 2022

Inappropriate DNA methylation of tumour suppressor genes can affect their expression and function, as such, has been a promising target for anti-cancer therapy. In gynaecological malignancy, ovarian cancer the highest associated rate mortality. This study investigated in vitro cytotoxicity four sesquiterpene lactones (SLs), including dehydroleucodine (Deh), alantolactone (Ala), costunolide (Cos...

Journal: :Food and Bioproducts Processing 2023

Biofouling in the food industry is a huge issue, and one possible way to reduce surface fouling understand how naturally cleaning surfaces based on biomimetic designs influence bacterial binding. Four self-cleaning leaves (Tenderheart cabbage, Cauliflower, White cabbage Leek) were analysed for their properties artificial replicates produced. The subjected attachment, adhesion retention assays u...

Journal: :Journal of Agricultural and Food Chemistry 2021

Development of efficient peptide-based immunotherapy for shrimp allergy relies on the identification dominant T-cell epitopes its major allergen, tropomyosin. In this study, immunoinformatic tools, proliferation, cytokine release, IgG/IgE binding, and degranulation assays were used to identify characterize in Lit v 1 comparison with previously validated B-cell epitopes. The results showed that ...

Journal: :Communications biology 2021

Abstract Members of the pentatricopeptide repeat (PPR) protein family act as specificity factors in C-to-U RNA editing. The expansion PPR superfamily plants provides sequence variation required for design consensus-based RNA-binding proteins. We used this approach to a synthetic editing factor target one sites Arabidopsis chloroplast transcriptome recognised by natural CHLOROPLAST BIOGENESIS 19...

Journal: :Bioorganic & medicinal chemistry 2013
Ramesh Alleti Josef Vagner Dilani Chathurika Dehigaspitiya Valerie E Moberg N G R D Elshan Narges K Tafreshi Nabila Brabez Craig S Weber Ronald M Lynch Victor J Hruby Robert J Gillies David L Morse Eugene A Mash

Probes for use in time-resolved fluorescence competitive binding assays at melanocortin receptors based on the parental ligands MSH(4), MSH(7), and NDP-α-MSH were prepared by solid phase synthesis methods, purified, and characterized. The saturation binding of these probes was studied using HEK-293 cells engineered to overexpress the human melanocortin 4 receptor (hMC4R) as well as the human ch...

Journal: :BMC Research Notes 2021

Abstract Objective We investigated the discrepancy between clinical and PCR-based diagnosis of COVID-19. compared results ten patients with mild to severe Respiratory samples from all cases were tested on Roche SARS-CoV-2 (Cobas) assay, Filmarray RP2.1 (bioMereiux) TaqPath™ COVID19 (Thermofisher) PCR assays. Results Laboratory records COVID-19 examined. Initially, respiratory as negative ® assa...

Journal: :Frontiers in virology 2023

Introduction The influenza virus genome consists of single-stranded RNAs and forms viral ribonucleoprotein (RNP) complexes. After replication in the nucleus, RNP interacts with protein M1. M1-viral complex is exported to cytoplasm via CRM1-dependent pathway using NS2/NEP as an export adaptor protein. NEP a 14 kDa diffusely localizes nucleus cytoplasm. Upon binding NLS motif M1, inhibits nuclear...

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