A technique has been described for the demonstration of a human complement component by an immunofluorescent method. The component detected is beta(1C)-globulin, a moiety of the third complement component, which has previously been obtained in pure form and to which a specific antiserum has been prepared. It has been shown in a model system that the binding of beta(1C)-globulin as shown by immu...

By ROBERT E. ANDERSON AND Ro L. WALFORD T HE PRESENT study forms part of a continuing effort to develop quantitative methods for detecting antigen-antibody reactions involving nucleated cells as antigen. Here we shall present information regarding the preparation of purified, 1131-labelled anti-human-globulin antibody (AHG antibody) by means of adsorption and elution from normal human globulin,...

By ROBERT E. ANDERSON AND Ro L. WALFORD T HE PRESENT study forms part of a continuing effort to develop quantitative methods for detecting antigen-antibody reactions involving nucleated cells as antigen. Here we shall present information regarding the preparation of purified, 1131-labelled anti-human-globulin antibody (AHG antibody) by means of adsorption and elution from normal human globulin,...

BACKGROUND Gc-globulin (vitamin D-binding protein) appears to have important functions in addition to its role as a carrier of vitamin D. APPROACH We reviewed recent studies focusing on the pathophysiologic functions and clinical significance of Gc-globulin. RESULTS Serum concentrations of Gc-globulin, as determined by immunoassay techniques, are decreased in severe injury. The extent of th...

Ren J., Song Ch.-L., Wang P., Li S., Kopparapu N.-K., Zheng X.-Q. (2015): Modification of structural and functional properties of sunflower 11S globulin hydrolysates. Czech J. Food Sci., 33: 474–478. The structural and functional properties such as solubility, emulsifying properties, foaming properties, oil binding capacity, and surface hydrophobicity of sunflower 11S globulin hydrolysates gene...

The effect of temperature on the binding of thyroxine and triiodothyronine to thyroxine-binding globulin has been studied by equilibrium dialysis. Inclusion of ovalbumin in the dialysis mixture stabilized thyroxine-binding globulin against losses in binding activity which had been found to occur during equilibrium dialysis. Ovalbumin by itself bound the thyroid hormones very weakly and its bind...