Two experiments were performed, each using six male and six female C57BL/6J mice collectively ranging from 4 wk to 17 mo of age. Blood was obtained following CO2 anesthesia, and the IL-10 concentration of each serum sample was determined both by sandwich enzyme-linked immunosorbent assay (ELISA) and by bioassay. In the first experiment, mean serum IL-10 immunoactivity was 9.3 pg/ml while the me...

An in vitro bioassay for mammalian thyroid stimulating hormone (TSH) based on TSH-induced cyclic adenosine 3',5'-monophosphate (cAMP) production in FRTL-5 cells, a rat thyroid cell line, was used to measure chicken TSH. The addition of chicken pituitary homogenate equivalent to > or = 25% of a chicken pituitary gland to cultured FRTL-5 cells increased cAMP within these cells in a dose-dependent...

Introduction: Bioassay is necessary for assessment of pollution. Because physical and chemical taste are not enough for assessment of potential effects on water ecosystem. Bioassay tests have alot of scientific value inanition doing of them is easy and need less time, also need less space, laboratory equipment’s and expenditure. Therefor such tests have found special attention in water pollutio...

In this article we report a simple and efficient method for detecting nonsteroidal estrogens in a biologic sample. This method uses polyclonal antibodies to estradiol (E2) to immunoprecipitate these major biologically active steroidal estrogens, leaving behind the nonsteroidal estrogens, which are then detected in a cell-based transcriptional activation bioassay for estrogen receptor agonist. T...

Many of the biological and toxic responses of polychlorinated dioxins and furans (PCDD/Fs) have been shown to be mediated through the aryl hydrocarbon receptor (AhR). Several bioanalytical methods have been developed to analyze Ah receptor agonists in cost-and-time effective way such as CALUX-bioassay (chemical-activated luciferase gene expression). This bioassay can detect the sum of dioxin-li...

An inexpensive and rapid bioassay using adult red flour beetles was developed for use in assessing the decontamination of environments containing organophosphates and related chemicals. A decontamination protocol was developed which demonstrated that 2 to 3 applications of 5% bleach solution were required to obtain nearly complete decontamination of malathion. The bioassay was also used to scre...

Gentamicin levels were determined in 100 serum specimens by a new latex agglutination inhibition card test, a radioimmunoassay (RIA), and a bioassay. Correlation coefficients determined by linear regression analysis demonstrated that the levels obtained by the latex agglutination inhibition card test had a high degree of correlation with the RIA and could be performed much faster and more econo...

If iochemical Substrate's and mechanisms conid be identified, Pfogresst might be wade in the detection and remedration of .certain learning and-memory disabilities. "Bemory -transfer" or "behavioral bioassay" methodology is a° new technique developed or this purpose. It uses the behavior recipient animals to' detect whateier chemicals are synthesized in -the braint of_ donor animals during lear...

A general field bioassay for detecting biologically active compounds in plants and insects has been developed and tested for efficacy and sensitivity. Methanolic extracts, in sucrose solution, of 20 plant and six caterpillar species were offered to the ponerine ant Paraponera clavata and the feeding preferences observed. The bioassay resulted in the detection of nine plant and three caterpillar...