Introduction The Pall Micro24 Bioreactor system is one of several microbioreactor systems that have been commercialised in recent years in response to the demand to reduce costs and shorten process development time lines. We have previously demonstrated that the Micro24 Bioreactor system can be integrated successfully into the later stages of cell line screening programmes and that the results ...

Process development, cheaper bioreactor cost, and faster fermentation rate can aid in reducing the cost of fermentation. In this article, these ideas were combined in developing a previously introduced textile bioreactor for ethanol production. The bioreactor was developed to utilize flocculating yeast for ethanol production under anaerobic conditions. A mixing system, which works without aerat...

Five crucian carp, Carassius auratus langsdorfiicarps had been reared in a closed water recirculating system. The system was equipped with the compact bioreactor using the plate gels capable of both nitrification and denitrification in a single unit. Ammonia and nitrite concentrations in the rearing water had been maintained below 0.05 mg-N/L, and nitrate concentration also controlled between 2...

A three-dimensional computational fluid dynamics- (CFD-) model based on a differential pressure laminar flow bioreactor prototype was developed to further examine performance under changing culture conditions. Cell growth inside scaffolds was simulated by decreasing intrinsic permeability values and led to pressure build-up in the upper culture chamber. Pressure release by an integrated bypass ...

The morphology of Ganoderma lucidum BCCM 31549 mycelium in a repeated-batch fermentation (RBF) was studied for exopolysaccharide (EPS) production. RBF was optimised for time to replace and volume to replace. G. lucidum mycelium showed the ability to self-immobilise and exhibited high stability for repeated use in RBF with engulfed pellets. Furthermore, the ovoid and starburst-like pellet morpho...

The key parameters of the inner structure of a cylindrical airlift bioreactor, including the ratio of the cross-section area of the downcomer to the cross-section area of the riser, clearance from the upper edge of the draft tube to the water level, and clearance from the low edge of the draft tube to the bottom of the reactor, significantly affected the biomass production of Botryococcus braun...

This paper reports the successful culture of Pueraria lobata (Willd.) suspension cells in a bioreactor. In vitro culture of this Chinese herb has potential as an alternative production method for industrial applications. Calli of P. lobata obtained from leaf explants were cultured in a 5.0 L bioreactor for two weeks. During this period, the pH of the medium declined from 5.8 to 4.5. By the end ...

Hydrolyzed wheat straw was converted into carboxylic acids and biohydrogen using digesting bacteria. The fermentations were carried out using both free and membrane-encased thermophilic bacteria (55°C) at various OLRs (4.42-17.95g COD/L.d), in semi-continuous conditions using one or two bioreactors in a series. The highest production of biohydrogen and acetic acid was achieved at an OLR of 4.42...

Surface-engineered yeast Saccharomyces cerevisiae codisplaying Rhizopus oryzae glucoamylase and Streptococcus bovis alpha-amylase on the cell surface was used for direct production of ethanol from uncooked raw starch. By using 50 g/L cells during batch fermentation, ethanol concentration could reach 53 g/L in 7 days. During repeated batch fermentation, the production of ethanol could be maintai...

The aim of this study was to investigate the feasibility of a bioreactor system and its scale-up to remove Cr(VI) from solution. The bioreactor is based on an innovative process that combines bioreduction of Cr(VI) to Cr(III) by the bacterium Arthrobacter viscosus and Cr(III) sorption by a specific zeolite. Batch studies were conducted in a laboratory-scale bioreactor, taking into account diffe...