In December 2009, the American Society for Veterinary Clinical Pathology (ASVCP) Quality Assurance and Laboratory Standards committee published the updated and peer-reviewed ASVCP Quality Assurance Guidelines on the Society's website. These guidelines are intended for use by veterinary diagnostic laboratories and veterinary research laboratories that are not covered by the US Food and Drug Admi...

A quantitative immunofluorescence platelet-associated immunoglobulins (PA-IgG) assay was used to detect alloimmunity to platelets in 8/12 multitransfused patients and to perform platelet crossmatching in the 8 alloimmunized patients. The correct separation of multitransfused patients into alloimmune and nonalloimmune groups was substantiated with chromium-51-labeled platelet survival studies. F...

Discrepancies in blood typing is one of the major reasons in eliciting a transfusion reaction. These discrepancies can be avoided through detailed analysis for the blood typing. Here, we report a subgroup of blood group type-B in the ABO system. Donor's blood was analyzed by employing commercial antisera for blood grouping. The results of forward (known antisera) and reverse (known antigen) rea...

Multi-center kidney paired donation (KPD) is an exciting new transplant option that has not yet approached its full potential. One barrier to progress is accurate virtual crossmatching for KPD waitlists with many highly sensitized patients. Virtual crossmatch results from a large multi-center consortium, the National Kidney Registry (NKR), were analyzed to determine the effectiveness of flexibl...

Detection of ABO blood groups through agglutination is the basis of pre-transfusion testing. However, weak agglutination reactions may be obtained with reagent antibodies and are a result of weak expression of A and B antigens on red blood cell surface which may cause a discrepancy in blood group typing. Here, we report a donor which showed discrepancy between red blood cells (forward) and seru...

The two-step matching process When an organ offer is made, a second cascade of screening events occurs. LifeLink Transplantation Immunology Laboratory in Tampa, FL, performs real-time nucleic-acid testing (NAT), which allows for the detection of HIV, West Nile virus, and hepatitis B genetic material. NAT is a more stringent protocol than simply testing the body’s response to an infectious disea...

A study of ABO blood group and Rh (D) factor, was carried out on 10478 cases. ‘0’ had the highest (35.13%) and blood group ‘AB’ the lowest (8.23%) frequency.Blood group 24.71% and 31.93%respectively. An overall 94.1 % of population was Rh (D)positive. Blood group A and B were A comparision of the ABO blood group and Rh(D) factor of the N.W.F.P., with that of Karachi population, shows that there...