Bone marrow transplantation (BMT) is a powerful strategy for the treatment of leukemia, aplastic anemia, congenital immunodeficiency and autoimmune diseases. In humans, bone marrow cells (BMCs) have usually been collected by multiple bone marrow aspirations from the iliac crest. We have established a new “perfusion” method for collecting BMCs with minimal contamination with the peripheral blood...

We have developed a powerful bone marrow transplantation method, intra-bone marrow-bone marrow transplantation (IBM-BMT), in which donor BM cells (BMCs) are directly injected into the recipient’s BM, resulting in a rapid recovery of donor hematopoiesis and a reduction in the severity of GVHD. We subsequently developed more effective BMT protocols by modifying IBM-BMT. One method involves the si...

introduction: quantification of epstein - barr virus (ebv) in peripheral blood mononuclear cells (pbmnc) of allogenic bone marrow transplant (bmt) recipients is important because ebv-associated posttransplant lymphoproliferative disease (ptld) can occur after transplantation due to immunosup-pression therapy. methods: to this end we chose real-time pcr using taqman probe. for the standard curve...

Advances in haploidentical bone marrow transplantation (h-BMT) have drastically broadened the treatment options for patients requiring BMT. The possibility of significantly reducing the complications resulting from graft-versus-host disease (GvHD) with the administration of post-transplant cyclophosphamide (PT-CY) has substantially improved the efficacy and applicability of T cell-replete h-BMT...

Impaired host defense post-bone marrow transplant (BMT) is related to overproduction of prostaglandin E(2) (PGE(2)) by alveolar macrophages (AMs). We show AMs post-BMT overproduce granulocyte-macrophage colony-stimulating factor (GM-CSF), whereas GM-CSF in lung homogenates is impaired both at baseline and in response to infection post-BMT. Homeostatic regulation of GM-CSF may occur by hematopoi...

Although rare cells expressing the bcr/abl fusion transcript can be detected by the polymerase chain reaction (PCR) in patient blood or marrow after allogeneic bone marrow transplant (BMT) for Philadelphia chromosome (Ph+)-positive chronic myelogenous leukemia (CML), the prognostic significance of this finding is unknown. This paper reports clinical, cytogenetic, and molecular data derived from...

Disease relapse and transplant related toxicities have limited the application of bone marrow transplantation (BMT) in the treatment for hematologic malignancies. Because elevated levels of tumor necrosis factor alpha (TNF-a) have been correlated with the development of transplant related complications, we conducted a phase 1-11 trial of pentoxifylline (PTX), a xanthine derivative capable of do...

84 INTRODUCTION I ntravenous bone marrow infusions have been reported as far back as 1939 (1) with curative attempts for hematologic disease dating to the 1950s (2–3). Since these early investigations, the field of bone marrow transplantation has experienced tremendous growth and advancement, especially in the last two decades. To date, there are over 200 transplant centers in the United States...

Human Herpes Virus 6 (HHV6) is known to be commonly associated with encephalitis related to immunosuppression and transplantation, and to bone marrow transplantation (BMT) in particular.1 HHV-6 is known to be common in transplant recipients and lead to several clinical manifestations, such as encephalitis.1-4 HHV 6 encephalitis in patients with BMT is known as latent infection. The first such c...