A method for preparing highly purified glutaminase (EC 3.5.1.2, L-glutamine amidohydrolase) from pig renal cortex is described. The main steps consist of sodium sulfate fractionation followed by alternative solubilization by dialysis against Tris-HCI buffer and precipitation with phosphateborate. The linal enzyme preparation contains no impurities which could be detected by sucrose gradient cen...

We present experimental investigations of electroosmotic pumping using porous borosilicate glass and various low ion density liquids. The orking liquids were deionized water, deuterium oxide (heavy water), methanol, acetone, acetonitrile and a 1 mM sodium borate buffer as a ontrol. We measured ionic conductivity and, as a useful comparative reference, the zeta potential generated by these liqui...

The detection and assay of vitamin B-2 (riboflavin) was accomplished under aqueous conditions using sodium borate buffering at pH 7.52 conditions. The absorbance spectrum of riboflavin was determined at different pH values utilizing several buffers. The buffer at pH at 7.52 is followed by accurate and sensitive assay of riboflavin by spectrophotometer at 440 nm wavelength. Where indicated an or...

A series of aldo-bis-indole derivatives (aldo-BINs) was prepared by aromatic C-alkylation reactions of aldoses and indole in acetic acid solution. Common monosaccharides such as glucose, mannose, galactose, fucose, xylose, rhamnose, ribose, arabinose and N-acetylglucosamine were smoothly derivatized to form the UV absorbing aldo-BINs. The use of a capillary electrophoretic method to separate th...

glutathione (gsh) is one of the most important antioxidants that plays an essential role in detoxification of reactive oxygen species (ros) which oxidizes to glutathione disulfide (gssg). paraquat (pq), awidely used herbicide, causes pulmonary injury with the productionof ros. excessive ros accumulation as a consequence of pq exposure are frequently targeted by gsh thereby oxidative stress lead...

Cu,Zn superoxide dismutase from baker's yeast, Saccharomyces cerevisiae, is inactivated by either butanedione in borate buffer or phenylglyoxal. Inactivation by butanedione takes place only in the presence of borate, and butanedione-inactivation is reversed on removal of borate. Initial studies with phenylglyoxal suggested that inactivation is due to the modification of 0.5 arginyl residues per...

L-Ribulose is a rare and expensive sugar that can be used as a precursor for the production of other rare sugars of high market value such as L-ribose. In this work we describe a production process for L-ribulose using L-arabinose, a common component of polymers of lignocellulosic materials, as the starting material. A ribulokinase-deficient mutant of the heterofermentative lactic acid bacteriu...

Although Mg2+ is an important cofactor for the specific degradation of RNA by ribozymes, it is not considered as a typical chemical nuclease. In this study we show that in combination with common buffers such as tris(hydroxymethyl)aminomethane and sodium borate, Mg2+ is a powerful catalyst for the degradation of RNA. pH and temperature are found to be the principal factors for the efficient deg...