This paper describes the development of a method for the simultaneous determination of ten anticoagulant rodenticides (coumafuryl, warfarin, pindone, coumatetralyl, coumachlor, difenacoum, bromadiolone, brodifacoum, chlorophacinone and flocoumafen) in the liver and kidney based on column-switching liquid chromatography coupled with heated electrospray ionization tandem mass spectrometry. The si...

Isolated five known iridoids glycosides (Scrophuloside A, Harpagoside B, 5-hydroxyloganin, 8-O-acetylharpagide and 6-O-methyl,1-glucopyranosyl catalpol), one phenyl ethanoid glycoside (Verbascoside) and a phenyl ethanol amine (2-(4-Chlorobenzyl amino) ethanol) compound from the methanolic extract of aerial parts of Scrophularia oxysepala using by high performance liquid chromatographyare based ...

A reversed-phase high performance liquid chromatography (HPLC) technique was developed for the analysis of sphingoid bases as their biphenylcarbonyl derivatives. The sphingoid bases (0.3-500 nmol) obtained from sphingolipids after hydrolysis were quantitatively derivatized with biphenylcarbonyl chloride at room temperature for 90 min. After removal of excess reagent by partitioning, the derivat...

Column selection in reversed-phase liquid chromatography is still not a straightforward process. In this study, we have evaluated and compared the performance of several traditional C18 phases with polar-modified phases and found distinct differences in the chromatographic behavior between two groups, as well as a high degree of variability within each group. The polar-endcapped phases exhibit ...

"Hydrophobic chromatography", which is a variation of reverse phase chromatography, is applicable to the analysis of cephalosporin C derivatives, especially in fermentation broths. Unfortunately, there are no commercial C18 columns which are entirely suitable for this class of compounds. For this reason C18 columns were prepared by an in-situ bonding technique and were optimally designed for ce...

A rapid and simple method is described for the preconcentration and discrimination between the organic fraction and the labile fraction of the metals Mn, Cd, Cu, Z, V, Ni, Pb, and Fe from seawater using 8-hydroxyquinoline, which forms neutral complexes with these metals, followed by adsorption on C18 chemically bonded silica gel. The sample flow rate through the C18 column was about 4 ml min. T...

This study was conducted to analyze carotenoids from tomatoes by high-performance liquid chromatography using reversed-phase C18 silica having cross-linked end-capping with diode array and mass spectrometric detection. An efficient gradient elution system was developed to achieve good and reliable separation of both major and minor carotenoids as well as their isomers. Resolution of lycopene, β...

Several isocratic separations for the determination of 20 steroids (STER) in animal feeding water samples (AFWS) from drinking-trough by LC using a mobile phase ACN/H(2)O (35:65 v/v) and different RP columns (Hypersil C18, Gemini C18 (GM), Purospher Star C18, Synergi Max C12, and Synergi Fusion) and UV detection were obtained. The elution order was the same: a first group of corticoids (CC) was...