Injection of steroid-free bovine follicular fluid (bFF; 2 \m=x\5 ml s.c. 12h apart) into anoestrous ewes lowered plasma FSH concentrations by 70% and after 24 h had significantly (P < 0\m=.\01)reduced the number of non-atretic follicles (\m=ge\1 mm diam.) without influencing the total number of follicles (\m=ge\1 mm diam.) compared to untreated controls. Hourly injections of FSH (10 \g=m\gi.v. ...

Inhibin-like activities in charcoal-treated bovine follicular fluid (FF) and medium from cultured Sertoli cells (SCCM) were assayed in an in nitro bioassay system, using cultured pituitary cells. Addition of both fluids resulted in parallel dose-dependent decreases of the concentration of follicle-stimulating hormone (FSH) in the medium, both in the presence or absence of luteinizing hormone-re...

The present study was aimed at determining whether insulin-like growth factor-1 (IGF-1) is a component of fetal bovine serum (FBS) that enables porcine cumulus cells to expand in response to FSH in vitro. Cumulus-oocyte complexes (COC) obtained from 4- to 6-mm follicles of prepubertal gilts were cultured at 39 degrees C for 24 h in media that contained human recombinant IGF-1 (50 ng/ml), FBS (1...

The objectives of the present studies by severalfold on d 4. In cells from both sizes of were to determine the effect of insulin, insulin-like follicles, insulin (1 pg/mL), in the presence of FSH, growth factor I (IGF-I), testosterone, and FSH on increased estradiol production per cell. In contrast, proliferation, progesterone production, and(or) esIGF-I (100 ng/mL) inhibited estradiol producti...

Patient A was a 30-year-old white woman with a longstanding history of primary amenorrhea and infertility. By age 18 the patient had neither menstruated nor demonstrated evidence of normal breast development. At that time she was given oral contraceptives for four cycles and responded with normal vaginal bleeding. Laboratory evaluation at age 18 demonstrated a serum FSH (follicle-stimulating ho...

A study was conducted to determine the effect of charcoal-extracted, bovine follicular fluid (CFF) on plasma follicle stimulating hormone (FSH) and luteinizing hormone (LH) concentrations, the interval from luteolysis to estrus, and subsequent luteal function in heifers. Fifteen Angus, Simmental and Hereford heifers were allotted by age, weight and breed to a control (C, n = 8) or a CFF (n = 7)...

Bovine preantral follicles (40-100 microm diameter at collection) were collected from ovaries of slaughtered cows and cultured in vitro with one of the four treatments: follicle stimulating hormone (FSH; 100 ng/ml) alone; FSH plus epidermal growth factor (EGF; 100 ng/ml); FSH plus insulin-transferrin-selenium (ITS; +1%) or FSH plus hypoxanthine (4 mM) in tissue culture medium (TCM 199) suppleme...

Interactions between mesenchymal-derived thecal cells and epithelial-derived granulosa cells are essential for follicular development in the ovary. These mesenchymal-epithelial cell interactions are in part mediated by keratinocyte growth factor (KGF), hepatocyte growth factor (HGF), and Kit ligand (KL). This study investigates the hypothesis that thecal cell-derived growth factors (e.g. KGF an...