The emergence and rapid spread of novel DS-1-like G1P[8] human rotaviruses in Japan were recently reported. More recently, such intergenogroup reassortant strains were identified in Thailand, implying the ongoing spread of unusual rotavirus strains in Asia. During rotavirus surveillance in Thailand, three DS-1-like intergenogroup reassortant strains having G3P[8] (RVA/Human-wt/THA/SKT-281/2013/...

The antigenic relationships between three bovine rotaviruses, UK, CP-1 and PP-1, and a porcine rotavirus were investigated; their pathogenicity for piglets was also assessed. After propagation and cloning in cell culture, the four viruses were used to produce convalescent and hyperimmune antisera in gnotobiotic animals. For the UK and PP-1 viruses, reciprocal virus neutralization tests gave rat...

The RNA segment encoding the guanylyltransferase (VP3) from 12 group A rotavirus isolates has been sequenced following RT-PCR and molecular cloning of the full-length amplicons produced. Alignment of the derived amino acid sequences including those of the four VP3 sequences available from GenBank revealed two levels of sequence divergence. Virus isolates from humans showed greater than 94% sequ...

AIMS Sewage systems are important nodes to monitor enteric pathogens transmitted via water. The aim of this study was to assess the presence of rotaviruses in effluents from wastewater treatment plants (WWTPs) and receiving streams in Beijing, China, to evaluate the reductions of rotaviruses in WWTPs and to provide viral fate and transport data for further epidemiological studies. METHODS AND...

Dilution of viruses, which were previously treated with 10 micrograms acetyltrypsin/ml, in MEM supplemented with 2.5% fetal bovine serum caused little damage of cell monolayers resulting in the formation of discrete plaques. When agar and acetyltrypsin were added to the overlay medium at the concentrations of 0.8% and 5 micrograms/ml, respectively, clear plaques were induced. Linear relationshi...

The distribution of the nonstructural protein 1 (NSP1) alleles from human strain AU-1 and canine strain K9 among rotaviruses of human, feline, canine, bovine, and simian origin was studied by a dot blot hybridization assay. Human and feline strains belonging to the AU-1 genogroup had the same NSP1 allele, while canine and feline strains belonging to the canine-feline genogroup shared another NS...

Neonatal diarrhea is the main cause of morbidity and mortality in calves, Rotavirus viral etiology. vaccines are one important methods for control neonates’ calves. In current study, Deoxyribonucleic acid (DNA) sequencing phylogenetic analysis Bovine Group a (BRVA) were performed our study. 1 Calf guard® vaccine genotype (G6P1) 5 different field genotypes (2 G6P5, G10P5, G10P? G10P11) subjected...