Myelinated fibers can be demonstrated in thin unstained brain sections of freshly killed uninjected animals. With this method myelinated fibers stand out as clearly as in stained Weigert sections from formalin-fixed brains. Unstained unfixed sections can be viewed under ordinary light within minutes after death of the animal and immediately photographed for permanent records. Such sections have...

All experimental models of traumatic brain injury (TBI) result in a progressive loss of brain tissue. The extent of tissue loss reflects the injury severity and can be measured to evaluate the potential neuroprotective effect of experimental treatments. Quantitation of tissue volumes is commonly performed using evenly spaced brain sections stained using routine histochemical methods and digital...

In the study of the pathology about 80 mice were examined. Half of these were studied in serial sections, cut at 10 microns, with every 15th section mounted and stained. The remainder were studied in non-serial sections. Hematoxylin-eosin and phloxinmethylene blue were used as routine, with silver and myelin stains where indicated. When infant mice were studied, the entire skull and brain were ...

Immunohistochemistry (IHC) is one of the most widely used laboratory techniques for the detection of target proteins in situ. Questions concerning the expression pattern of a target protein across the entire brain are relatively easy to answer when using IHC in small brains, such as those of rodents. However, answering the same questions in large and convoluted brains, such as those of primates...

PURPOSE We evaluated the intraobserver and interobserver variability in measuring long-term changes in the volume of brain lesions on 5- and 3-mm-thick MR sections in patients with multiple sclerosis. METHODS Eighteen 18 patients were scanned on two separate occasions with a mean interval of 16.4 months between the two examinations. In each session, a scan with 24 contiguous 5-mm-thick axial ...

Intracerebral hemorrhage (ICH) is a fatal stroke subtype with significant public health impact. Although neuroinflammation is a leading cause of neurological deficits after ICH, no imaging tool is currently available to monitor brain inflammation in ICH patients. Given the role of TSPO in neuroinflammation, herein we investigate whether a second-generation TSPO ligand, [125 I]IodoDPA-713 can be...

Twenty-two normal preterm and 10 full-term infants were studied by cranial computed tomography. The study included correlation of the gestational age and birth weight to the ventricular size, brain size, and the area of the sylvian fissure. The most relevant parameters were the bifrontal diameter, bifrontal index, and the transverse diameter of the brain. The incidence of visualization of the s...